Effect of dexamethasone sodium phosphate nanomaterials on IL-1β-induced inflammation in primary rat chondrocytes
Objective:To explore the effect of dexamethasone(Dex)sodium phosphate nanomaterials(Nano-Dex)on interleukin(IL)-1β-induced inflammation in primary rat chondrocytes.Methods:Transmission electron microscopy was used to observe the morphology of Nano-Dex nanoparticles;Malvern particle size meter was used to detect the particle size as well as the potential of Nano-Dex.SD rat chondrocytes were cultured,and the cells were randomly divided into blank group,IL-1β group,IL-1β+Dex group,and IL-1β+Nano-Dex group.The expression levels of matrix metalloproteinase(MMP)13,MMP3,IL-1β,IL-6,collagen type Ⅱ alpha 1(Col2a1)and proteoglycan(ACAN)genes in chondrocytes were detected by reverse transcription-quantitative PCR(RT-qP-CR).The expression of MMP13 was observed by immunofluorescence staining.The expression levels of MMP13,Col2a1,IL-6,IL-1β,P65 and Caspase 3 in chondrocytes were detected by western blotting.Results:Na-no-Dex had a rounded shape,uniform size,diameter of about 90 nm,and a positive charge.Compared with the blank group,the expression of cartilage repair genes Col2a1 and ACAN in the IL-1β group was down-regulated,while the expression of inflammatory genes IL-1β,IL-6,MMP3 and MMP13 was up-regulated(all P＜0.05).Compared with the IL-1β group,the expression of Col2a1 gene and protein as well as the expression of ACAN gene in the Nano-Dex+IL-1β group were up-regulated,and the expression of IL-1β,IL-6,MMP3 and MMP13 genes as well as the expression of MMP13,P65,IL-6,IL-1β and Caspase 3 proteins were down-regulated(all P＜0.05).Immunofluorescence results showed that the fluorescence intensity of MMP13 in the IL-1β +Nano-Dex group was significantly lower than that in the IL-1β group(P＜0.05).Conclusion:Nano-Dex can effectively in-hibit IL-1β-induced chondrocyte inflammation.
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