Mechanism of RASA4 expression inactivation promoting proliferation of nasopharyngeal car-cinoma
Objective:To explore the expression of RASA4gene in nasopharyngeal carcinoma(NPC),and to in-vestigate the influence and molecular mechanism of RASA4gene affecting the proliferation of NPC.Methods:Meta-analysis of RASA4transcription levels in NPC was performed using GEO database.RASA4mRNA and protein expression levels in NPC cell lines were detected by reverse transcription-quantitative PCR(RT-qPCR)and western blotting.RASA4protein expression and localization in NPC tissues were detected using immunohis-tochemistry(IHC).The impact of RASA4on cell proliferation was evaluated through cell counting kit-8(CCK-8)assay and colony formation assay.Results:Bioinformatics analysis based on GEO data showed that RASA4 mRNA expression was significantly down-regulated in NPC.RT-qPCR and western blotting results indicated that RASA4 was down-regulated at both mRNA and protein expression in NPC cells.Exogenous overexpression of the RASA4gene could inhibit the cell proliferation of NPC.Conclusion:RASA4may serve as a potential sup-pressor gene in NPC,and investigating its inactivation mechanism and function is of significant importance for the pathogenesis and treatment of NPC.
国家科技期刊平台
NSTL
万方数据
