Effect of palladium-zinc alloy nanozymes on an inflammatory chondrocyte model
Objective:To discuss the anti-inflammatory effect of palladium-zinc alloy(PdZn/C)nanozymes on an inflammatory chondrocyte model induced by hydrogen peroxide(H2O2).Methods:The PdZn/C nanozymes were synthesized and characterized.The PdZn/C nanozymes were assayed for simulated superoxide dismutase ac-tivity(SOD)and simulated catalase activity(CAT).The biocompatibility of PdZn/C nanozymes with chondro-cytes was examined in vitro by cell counting kit-8(CCK-8)assay.The H2O2-induced inflammatory chondrocytes were co-cultured with PdZn/C nanozymes,the reactive oxygen species(ROS)levels of inflammatory chondro-cytes were assessed by ROS fluorescent probe,and immunofluorescence assay was performed to detect the pro-tein expression levels of interleukin(IL)-1β and tumor necrosis factor(TNF)-α in inflammatory chondrocytes.Re-sults:The successful synthesis of PdZn/C nanozymes was confirmed by transmission electron microscopy(TEM),X-ray diffraction(XRD)and zeta potential.The results of CCK-8 assay confirmed that PdZn/C nano-zymes had good biocompatibility.The PdZn/C nanozymes had excellent SOD and CAT mimicking activities.The PdZn/C nanozymes were able to reduce the ROS levels as well as the expression of IL-1β and TNF-α proteins in the inflammation model.Conclusion:The PdZn/C nanozymes have good biocompatibility and can reduce the in-flammatory response of chondrocytes,making them a promising anti-inflammatory nanomaterial.
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