The significance of circular RNA hsa_circ_0006156 expression in peripheral blood of SLE pa-tients and its effect on proliferation and apoptosis of Raji cells
Objective:To investigate the effect of hsa_circ_0006156 on the proliferation and apoptosis of Raji cells and its role in the occurrence and development of systemic lupus erythematosus(SLE).Methods:A total of 55 SLE patients(SLE group)admitted to the Department of Dermatology and Venereal Diseases,the First Affili-ated Hospital of Guangxi Medical University from September 2021 to September 2023 were collected,and 40 healthy controls were taken as the control group(HC group).Peripheral blood mononuclear cells(PBMC)and CD20+B lymphocytes were isolated.Reverse transcription-quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression of hsa_circ_0006156,and receiver operating characteristic(ROC)curve was used to analyze the diagnostic efficacy of hsa_circ_0006156.Taking Raji cells as the research object,the hsa_circ_0006156 overexpression model was constructed by lentivirus.The cells were divided into hsa_circ_0006156 overexpression group(OE group),negative control group(NC group)transfected with empty viral vec-tor,and blank control group(control group).CCK8 assay was used to detect the cell absorbance of 450 nm at 0 h,6 h,12 h,18 h,24 h,30,36 h,and the proliferation rate of OE group and NC group was calculated.Western blot-ting was used to detect the expression levels of Bcl-2 and cleaved caspase-3 apoptotic proteins in each group.Re-sults:Compared with the HC group,the expression of hsa_circ_0006156 in PBMC and CD20+B lymphocytes in the SLE group was significantly decreased(P＜0.001).ROC curve analysis showed that the area under the ROC curve(AUC)of hsa_circ_0006156 distinguishing SLE and HC was 0.7873(95%CI:0.6975-0.877;P＜0.001),sensitivity was 97.5%,specificity was 50.9%,and cut-off value for SLE diagnosis was less than 0.652.There was a significant difference in the expression of hsa_circ_0006156 between patients with no or mild activity and mod-erate to severe activity(P=0.0148).CCK8 assay showed that the proliferation of Raji cells in the OE group was significantly inhibited compared with the NC group at 36 h(P＜0.001).The results of western blotting showed that compared with the NC group and the control group,the expression level of Bcl-2 protein in the OE group was decreased,while the expression level of cleaved caspase-3 protein was increased(all P＜0.05).Conclusion:The hsa_circ_0006156 has certain diagnostic efficacy for SLE.It may affect the occurrence and development of SLE by regulating the proliferation and apoptosis of B cells.
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