首页|裂果薯总皂苷对大鼠肝卵圆细胞系WB-F344上皮间质转化的影响

裂果薯总皂苷对大鼠肝卵圆细胞系WB-F344上皮间质转化的影响

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的:探讨裂果薯总皂苷(SSPHs)对转化生长因子-β1(TGF-β1)诱导的大鼠肝卵圆细胞系WB-F344上皮间质转化(EMT)的影响及作用机制.方法:将WB-F344细胞分为对照组、模型组,SSPHs低、中、高剂量组和PI3K/AKT信号通路抑制剂LY294002组.除对照组外,其余各组均以10 μg/L TGF-β1诱导WB-F344构建EMT模型.采用MTT法、细胞划痕实验分别检测细胞增殖、迁移能力,实时荧光定量PCR(RT-qPCR)、western blotting法分别检测E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)的mRNA和蛋白表达,western blotting法检测PI3K/AKT信号通路关键蛋白表达.结果:SSPHs可抑制TGF-β1诱导的WB-F344细胞的增殖,24 h的细胞半数抑制浓度(IC50)为3.02 μg/mL.与对照组比较,模型组N-cad-herin、Vimentin的mRNA及蛋白表达水平显著升高,E-cadherin mRNA及蛋白表达水平显著降低,p-PI3K/PI3K、p-AKT/AKT比值显著升高(均P＜0.05).与模型组比较,SSPHs中、高剂量组N-cadherin和Vimentin mRNA及蛋白表达下调,E-cadherin mRNA及蛋白表达上调,p-PI3K/PI3K、p-AKT/AKT比值降低(均P＜0.05),结果与LY294002组相似.结论:SSPHs可抑制TGF-β1诱导的WB-F344细胞EMT进程,其机制可能与抑制PI3K/AKT信号通路有关.
The effect of total saponins of Schizocapsa plantaginea Hance on epithelial-mesenchymal transition of rat hepatic oval cell line WB-F344
Objective:To investigate the effect and mechanism of total saponins of Schizocapsa plantaginea Hance(SSPHs)on epithelial mesenchymal transformation(EMT)of rat hepatic oval cells WB-F344 induced by transforming growth factor-β1(TGF-β1).Methods:WB-F344 cells were divided into control group,model group,low-,medium-and high-dose SSPHs groups and PI3K/AKT signaling pathway inhibitor LY294002 group.Except the control group,all the other groups were induced with 10 μg/L TGF-β1 to establish the EMT model.MTT assay and cell scratch assay were used to detect cell proliferation and migration.Reverse transcrip-tion-quantitative PCR(RT-qPCR)and western blotting were used to detect the mRNA and protein expression of E-cadherin,N-cadherin and Vimentin,respectively.The expression of key proteins in PI3K/AKT signaling path-way was detected by western blotting.Results:SSPHs inhibited the proliferation of TGF-β1-induced WB-F344 cells,with a half maximal inhibitory concentration(IC50)value of 3.02 μg/mL at 24 hours.Compared with the control group,the model group showed significantly increased mRNA and protein expression levels of N-cad-herin and Vimentin,significantly decreased mRNA and protein expression of E-cadherin,and significantly in-creased ratio of p-PI3K/PI3K and p-AKT/AKT(all P＜0.05).Compared with the model group,N-cadherin and Vimentin mRNA and protein expression was down-regulated,E-cadherin mRNA and protein expression was up-regulated,and P-PI3K/PI3K and P-AkT/AKT ratios were decreased in the THE SSPHs medium and high-dose groups(all P＜0.05),with results similar to those observed in the LY294002 group(all P＜0.05).Conclusion:SSPHs can inhibit the EMT process in TGF-β1-induced WB-F344 cells,and its mechanism may be related to the inhibition of PI3K/AKT signaling pathway.

total saponins of Schizocapsa plantaginea Hancehepatic oval cellsPI3K/AKTepithelial-mesen-chymal transitionhepatic precancerous lesiontransforming growth factor-β1

李锦华、莫宇雪、郭心怡、梁梓樱、李昊、吕美娴、王燕雪、梁钢

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广西医科大学药学院,南宁 530021

省部共建靶向肿瘤学国家重点实验室,南宁 530021

裂果薯总皂苷 肝卵圆细胞 PI3K/AKT 上皮间质转化 肝癌前病变 转化生长因子-β1

国家自然科学基金资助项目国家自然科学基金资助项目

8196073782360792

2024

10.16190/j.cnki.45-1211/r.2024.05.001

广西医科大学学报
广西医科大学

广西医科大学学报

CSTPCD
影响因子：0.788
ISSN：1005-930X
年,卷(期)：2024.41(5)