首页|铜代谢相关基因COX17与头颈部鳞状细胞癌不良预后相关

铜代谢相关基因COX17与头颈部鳞状细胞癌不良预后相关

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的:探讨铜代谢相关基因细胞色素c氧化酶铜伴侣17(COX17)对头颈部鳞状细胞癌(HNSCC)发生发展及预后的影响.方法:运用生物信息学分析COX17在HNSCC与正常组织中的表达差异;通过构建列线图验证COX17在预测HNSCC预后中的作用;采用KEGG和GO分析对COX17相关基因进行功能富集分析以及利用ssGSEA分析COX17表达与HNSCC组织中免疫细胞浸润丰度的关系;采用实时定量聚合酶链反应(RT-qPCR)验证COX17在HNSCC细胞系与永生化正常上皮细胞系中的表达差异;细胞活力试剂盒(CCK-8)和Transwell实验分别用于检测COX17对细胞增殖、侵袭能力的影响.结果:COX17在HNSCC中表达显著上调(P＜0.05).预后分析表明COX17与HNSCC患者总生存期(OS)密切相关(P＜0.05).免疫浸润相关性分析显示,COX17与多种免疫细胞浸润丰度呈负相关关系(均P＜0.001).敲低COX17可显著抑制HNSCC细胞SCC-9、SAS细胞的增殖及侵袭能力.结论:COX17的表达可影响HNSCC细胞的增殖和侵袭,并与HNSCC组织中免疫细胞浸润存在潜在关联;作为线粒体铜代谢分子标记物,COX17可能是评估HNSCC预后的有效指标和潜在治疗靶点.
The copper metabolism-related gene COX17 is associated with poor prognosis of head and neck squamous cell carcinoma
Objective:To investigate the impact of copper metabolism-related gene cytochrome c oxidase copper chaperone 17(COX17)on the occurrence,development and prognosis of head and neck squamous cell carcino-ma(HNSCC).Methods:Bioinformatics analysis was employed to assess the expression differences of COX17 between HNSCC and normal tissues.Nomogram was generated to verify the role of COX17in predicting the prognosis of HNSCC.Functional enrichment analysis of COX17-related genes was conducted using KEGG and GO analyses,and the relationship between COX17expression and the abundance immune cell infiltration in HN-SCC tissues was analyzed using ssGSEA.Reverse transcription-quantitative polymerase chain reaction(RT-qP-CR)was utilized to verify the expression differences of COX17between HNSCC cell lines and immortalized nor-mal epithelial cell lines.The effect of COX17on cell proliferation and invasion was assessed using the cell count-ing kit-8(CCK-8)assay and Transwell experiments,respectively.Results:COX17expression was significantly up-regulated in HNSCC(P＜0.05).Prognostic analysis indicated that COX17expression was closely associated with the overall survival(OS)of HNSCC patients(P＜0.05).Immune infiltration correlation analysis revealed a significant negative correlation between COX17expression and the abundance of various immune cell infiltration(all P＜0.001).Knocking down COX17significantly inhibited the proliferation and invasion abilities of HNSCC cell line SCC-9 and SAS.Conclusion:The expression of COX17can affect the proliferation and invasion of HN-SCC cells and is potentially associated with immune cell infiltration in HNSCC tissues.As a biomarker of mito-chondrial copper metabolism,COX17may be an effective indicator for assessing the prognosis of HNSCC and a potential therapeutic target.

cytochrome c oxidase copper chaperone 17copper metabolismhead and neck squamous cell carci-nomagrowthinvasionprognosis

卢苇、江川、何馨露、姚茜、苏敬雅、刘春磊、谢莹、韦正波

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广西医科大学附属肿瘤医院,南宁 530021

广西区域性高发肿瘤早期防治研究重点实验室,南宁 530021

广西医科大学生命科学研究院,南宁 530021

细胞色素c氧化酶铜伴侣17 铜代谢 头颈鳞癌 生长 侵袭 预后

国家自然科学基金资助项目广西自然科学基金资助项目广西自然科学基金资助项目

821603862021GXNSFAA0750422023GXNSFAA026189

2024

10.16190/j.cnki.45-1211/r.2024.05.008

广西医科大学学报
广西医科大学

广西医科大学学报

CSTPCD
影响因子：0.788
ISSN：1005-930X
年,卷(期)：2024.41(5)