Evaluation of cardiopulmonary ultrasound on progression and efficacy of doxorubicin-in-duced heart failure in rats
Objective:To explore the evaluation effect of cardiopulmonary ultrasound(CPUS)on the progres-sion and efficacy of doxorubicin(DOX)-induced heart failure(HF)rats.Methods:Eighty-eight SD rats were ran-domly divided into three groups:control group(n=8),model group(n=48,intraperitoneal injection of DOX,twice a week),and treatment group(n=32,6 weeks after DOX injection,Qili Qiangxin Capsules were given intra-gastric administration,once a day).The model group was divided into 6 subgroups with 8 rats in each group ac-cording to the duration of DOX injection(1-6 weeks).The treatment group was divided into 4 subgroups with 8 rats in each group according to the duration of intragastric administration(1-4 weeks).CPUS and histopathologi-cal examination were performed in all groups.Results:Compared with the control group,the left atrioventricular chamber in the model group enlarged;the left atrial diameter(LAD)at the end of the contraction,the ratio of left atrial diameter to right atrial diameter(LAD/RAD)at the end of the contraction,and the ratio of left ventricular end-diastolic diameter to right ventricular end-diastolic diameter(LVEDD/RVEDD)were all higher than those in the control group;the E/e'values of both the left and right ventricles were increased;the left ventricular ejection fraction(LVEF)and the tricuspid annular plane systolic excursion(TAPSE),and the left ventricular wall thick-ness(LVWT)were decreased(all P＜0.05).In the model group,the pleural line and A-lines were blurred or dis-appeared,the number of B-lines was increased,and the lung ultrasound score(PLUS)was higher than that of the control group.HE staining showed that the histopathological changes in the heart of the model group were myo-cardial cell degeneration and edema,with disordered myocardial fiber arrangement,interstitial edema and porosi-ty,and inflammatory cell infiltration.The histopathological changes in the lung tissue were destruction of alveo-lar structure,thickening of lobular septa,effusion in alveolar cavity,congestion of alveolar capillaries,and inflam-matory cell infiltration,which were consistent with the histopathological changes of heart and lung in HF.In the treatment group,the left atrioventricular chamber was decreased,LAD and LVEDD/RVEDD were lower than those in the model group(P＜0.05),while there was no statistically significant difference in LAD/RAD(P＞0.05).The systolic and diastolic functions of the left and right ventricles were improved,LVEF and TAPSE were higher than those in the model group,and the E/e'values of both the left and right ventricles were lower than those in the model group(P＜0.05).LVWT was higher than that in the control group,while RVWT was lower than that in the control group(P＜0.05).The lung ultrasound showed a decrease in the number of B-lines,and A-lines were visible.Compared with the model group injected for 6 weeks,the PLUS score of the treatment group was significantly lower after 3 and 4 weeks of injection(P＜0.05),and the pathological morphology of the heart and lung gradually improved with the extension of the administration time.There was a significant correlation be-tween cardiac ultrasound parameters and PLUS.LAD and LVEDD/RVEDD were positively correlated with PLUS,while LVEF and TAPSE were negatively correlated with PLUS(P＜0.001).Conclusion:CPUS can dy-namically monitor the disease progression of HF rats,evaluate the effectiveness of intervention and treatment,and closely track the progression of the disease,which is expected to provide effective monitoring methods for ba-sic research and clinical treatment of HF.
heart failuredoxorubicincardiopulmonary ultrasoundanimal model
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