摘要
目的:通过比较活化T细胞核因子胞质4(NFATc4)与S100钙结合蛋白(S100)、p75神经营养素受体(p75)对舌鳞状细胞癌(TSCC)神经侵犯(PNI)的免疫组织化学染色特点,探索NFATc4在TSCC PNI诊断中的作用.方法:收集59例TSCC病理标本,10例癌前病变为对照组,每个标本连续切片后采用免疫组织化学染色,观察NFATc4对TSCC以及神经的染色情况,并与S100和p75进行比较.结果:59例TSCC病理标本中,NFATc4阳性率为47.5%(28/59),PNI发生率为35.6%(21/59),NFATc4阳性表达组的PNI发生率高于NFATc4阴性表达组(P＜0.05),NFATc4染色可见神经内膜淡染色,TSCC细胞胞质可见染色,NFATc4对神经的鉴别效果与S100和p75比较,无统计学差异(P＞0.05).结论:NFATc4的表达与PNI的发生存在关联,NFATc4能在染色神经束的同时,将TSCC组织染色,可以在同一个视野内直观地观察肿瘤与神经的关系,有利于提高PNI判读准确率,有望成为一个判断PNI的指标.
Abstract
Objective:To explore the role of activated T-cell nuclear factor cytoplasmic 4(NFATc4)in the diag-nosis of perineural invasion(PNI)in tongue squamous cell carcinoma(TSCC)by comparing the immunohisto-chemical staining characteristics of NFATc4 with those of S100 calcium binding protein(S100)and p75 neuro-trophin receptor(p75)for PNI in TSCC.Methods:A total of 59 pathological specimens of TSCC and 10 precan-cerous lesions were collected as control group.Each specimen underwent serial sectioning and immunohisto-chemical staining to observe the staining of TSCC as well as nerves by NFATc4.These staining patterns were then compared with those of S100 and p75.Results:In the 59 TSCC pathological specimens,the positivity rate of NFATc4 was 47.5%(28/59),and the PNI incidence was 35.6%(21/59).The PNI incidence was higher in the NFATc4 positive expression group than that in the NFATc4 negative expression group(P＜0.05).NFATc4 stain-ing was observed as light staining of the nerve endothelium and staining of the TSCC cell cytoplasm.The identifi-cation results of NFATc4 of nerves were not statistically different from those of S100 and p75(P＞0.05).Conclu-sion:There is an association between NFATc4 expression and the occurrence of PNI.NFATc4 can stain the TSCC tissues while staining the nerve bundles,which makes it possible to visualize the relationship between the tumor and the nerves in the same field of view.This visualization enhances the diagnostic accuracy of PNI,indicating that NFATc4 can potentially serve as a diagnostic indicator for PNI.
基金项目
广西自然科学基金资助项目(2016GXNSFDA380002)
国家科技期刊平台
NSTL
万方数据