Effect of ALDH2 gene expression on ketamine-induced cystitis based on oxidative stress-me-diated NF-κB pathway
Objective:To investigate the effect of acetaldehyde dehydrogenase 2(ALDH2)gene expression on oxidative stress-mediated NF-κB pathway in ketamine-induced cystitis(KIC).Methods:SV-HUC-1 cells were divided into different concentrations of ketamine groups(0 mmol/L,0.5 mmol/L,1 mmol/L,2 mmol/L),and the expression of ALDH2 protein was detected after induction for 48 hours.The SV-HUC-1 cell constructs were di-vided into control group(si-NC group,OE-NC group),ALDH2 silencing group(Si-ALDH2 group),and ALDH2 overexpression group(OE-ALDH2 group).The cells were induced with complete medium and ketamine(1 mmol/L)for 48 hours respectively,and the levels of inflammatory factors and inflammatory proteins were detected in each group.Results:Western blotting showed that the relative expression of ALDH2 protein after treatment with ketamine(0.5 mmol/L,1 mmol/L)was significantly higher than that in the 0 mmol/L group(P＜0.05).In the fur-ther comparison of ketamine-induced group(1 mmol/L),it was found that the expression of inflammatory cyto-kines interleukin-1β(IL-1β)and interleukin-6(IL-6)in the si-ALDH2 group was significantly higher than that in the si-ALDH2 group(P＜0.05);the expression of IL-1β and IL-6 in the OE-ALDH2 group was significantly low-er than that in the OE-NC group(P＜0.05).The expression levels of inflammatory protein nuclear factor-κB(NF-κB),cyclooxygenase-2(COX-2)and inducible nitric oxide synthase(iNOS)in the si-ALDH2 group were higher than those in the si-NC group(P＜0.05).The expression levels of inflammatory proteins NF-κB,COX-2 and iN-OS in the OE-ALDH2 group were lower than those in the OE-NC group(P＜0.05).In the control group(0 mmol/L),there were no significant differences in the levels of inflammatory factors and inflammatory proteins between si-ALDH2 group and si-NC group,as well as OE-ALDH2 group and OE-NC group(P＞0.05).Conclusion:AL-DH2gene may play a certain anti-inflammatory protective role in the occurrence of KIC.
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