Effect of total saponins of Schizocapsa plantaginea Hance on epithelial mesenchymal transfor-mation,migration and invasion of WB-F344 malignant transformed cells
Objective:To investigate the effect and mechanism of total saponins of Schizocapsa plantaginea Hance(SSPHs)on epithelial mesenchymal transformation(EMT),migration and invasion of WB-F344 malig-nant transformed cells.Methods:A malignant transformation cell model was prepared by inducing rat hepatic oval cell line WB-F344 in vitro using the carcinogenic agent 1-methyl-3-nitro-1-nitrosoguanidine(MNNG).The experiment was divided into control group,MNNG group,MNNG+SSPHs group,MNNG+Wnt-1/β-catenin sig-naling pathway activator lithium chloride(LiCl)+signaling pathway inhibitor(XAV-939)group,MNNG+LiCl group,and MNNG+LiCl+SSPHs group.Cell counting kit-8(CCK-8)assay was used to detect the activity of SSPHs on WB-F344 malignant transformed cells;cell scratch assay and Transwell assay were used to detect the migration and invasion ability of WB-F344 malignant transformed cells,respectively.The expression of E-cad-herin,N-cadherin,Vimentin,Wnt-1 and β-catenin proteins was detected by western blotting.Results:After SSPHs treatment,the activity of malignant transformed cells of WB-F344 was inhibited(P＜0.05).Compared with the control group,the MNNG group showed an increased scratch healing rate,an increased number of cell membrane penetration,elevated expression levels of N-cadherin,Vimentin,Wnt-1,β-catenin proteins,and a de-creased expression level of E-cadherin protein(all P＜0.05).Compared with the MNNG group,the MNNG+SSPHs group showed a decrease in scratch healing rate,a decrease number of membrane penetration,down-regu-lation of N-cadherin,Vimentin,Wnt-1 and β-catenin protein expression,and up-regulation of E-cadherin protein expression(all P＜0.05).Compared with the MNNG+LiCl group,the protein expression of N-cadherin,Vimen-tin,Wnt-1 and β-catenin in the MNNG+LiCl+SSPHs group was down-regulated,while the protein expression of E-cadherin was up-regulated(all P＜0.05),and the results were the same as those of MNNG+LiCl+XAV-939 group.Conclusion:SSPHs can inhibit EMT,migration and invasion of WB-F344 malignant transformed cells,and the mechanism may be related to the regulation of Wnt-1/β-catenin signaling pathway.
total saponins of Schizocapsa plantaginea HanceWnt-1/β-catenin signaling pathwayWB-F344 ce-llsmalignant transformationepithelial mesenchymal transformationmigrationinvasion
万方数据
维普
