Value of quantitative evaluation of contrast-enhanced ultrasound on the anti-angiogenesis by combining cisplatin with curcumin in the treatment of laryngopharyngeal carcinoma xeno-grafts
Objective:To explore the application value of contrast-enhanced ultrasound(CEUS)quantitative analysis in evaluating the chemotherapeutic effect and anti-angiogenesis influence of cisplatin combined with cur-cumin in nude mice of laryngopharyngeal carcinoma xenograft model.Methods:The nude mouse xenograft mod-els of human laryngopharyngeal carcinoma FaDu cell line were established and randomly divided into normal sa-line group,sodium carboxymethyl cellulose(CMC-Na)group,cisplatin group,curcumin group and cisplatin com-bined with curcumin group(combined group).Tumor size before and after administration was compared,the tu-mor inhibition rate was calculated,CEUS and quantitative analysis were conducted to obtain blood perfusion pa-rameters,including peak intensity(PI),area under the curve(AUC),and time to peak(TTP).The tumor microves-sel density(MVD)was detected by immunohistochemical staining,and the correlation between CEUS quantita-tive parameters and MVD and tumor volume was analyzed by Pearson correlation analysis.Results:The tumor volume in the cisplatin group,curcumin group and combined group was smaller than that in the normal saline group and CMC-Na group(P＜0.05),and the tumor volume in the combined group was the smallest(P＜0.05).The tumor inhibition rates of cisplatin group,curcumin group and combined group were 43.62％,37.28％and 65.42％,respectively.The CEUS quantitative parameters PI and AUC in the cisplatin group,curcumin group and combined group were lower than those in the normal saline group and CMC-Na group(P＜0.05).MVD in the cis-platin group,curcumin group and combined group was lower than that in the normal saline group and CMC-Na group(P＜0.05),and MVD in the combined group was the lowest(P＜0.05).MVD was positively correlated with PI,AUC and tumor volume(r=0.937,0.931,0.812,all P＜0.05).PI and AUC were positively correlated with tumor volume(r=0.807,0.795,P＜0.05).Conclusion:Cisplatin combined with curcumin can enhance the growth inhibition of human laryngopharyngeal carcinoma xenografts in nude mice.CEUS quantitative parame-ters PI and AUC can effectively reflect tumor micro-angiogenesis and evaluate the chemotherapy efficacy of la-ryngopharyngeal carcinoma xenografts.
laryngopharyngeal carcinomacontrast-enhanced ultrasoundcisplatincurcuminmicrovessel density
万方数据
维普
