摘要
目的:探讨超声造影(CEUS)定量分析评价顺铂联合姜黄素治疗裸鼠喉咽癌移植瘤的化疗效果及对血管生成作用的应用价值.方法:建立人喉咽癌FaDu细胞株裸鼠皮下移植瘤模型,随机分为生理盐水组、羧甲基纤维素钠(CMC-Na)组、顺铂组、姜黄素组、顺铂联合姜黄素组(联合组).比较给药前、后各组肿瘤大小,计算抑瘤率,CEUS定量分析获取峰值强度(PI)、曲线下面积(AUC)、达峰时间(TTP)等血流灌注参数,免疫组织化学染色检测肿瘤微血管密度(MVD).采用Pearson相关分析法分析CEUS定量参数与MVD及移植瘤体积的相关性.结果:顺铂组、姜黄素组、联合组移植瘤体积较生理盐水组和CMC-Na组缩小(P＜0.05),且联合组移植瘤体积最小(P＜0.05);顺铂组、姜黄素组、联合组抑瘤率分别为43.62％、37.28％、65.42％.顺铂组、姜黄素组、联合组CEUS定量参数PI、AUC低于生理盐水组和CMC-Na组(P＜0.05);顺铂组、姜黄素组、联合组MVD低于生理盐水组和CMC-Na组(P＜0.05),且联合组MVD最低(P＜0.05).MVD与PI、AUC、肿瘤体积均呈正相关关系(r=0.937、0.931、0.812,均P＜0.05),PI、AUC与肿瘤体积呈正相关系统(r=0.807、0.795,P＜0.001).结论:顺铂联合姜黄素可增强对人喉咽癌裸鼠移植瘤的生长抑制作用;CEUS定量参数PI、AUC可反映肿瘤微血管生成,并能有效评价喉咽癌移植瘤的化疗效果.
Abstract
Objective:To explore the application value of contrast-enhanced ultrasound(CEUS)quantitative analysis in evaluating the chemotherapeutic effect and anti-angiogenesis influence of cisplatin combined with cur-cumin in nude mice of laryngopharyngeal carcinoma xenograft model.Methods:The nude mouse xenograft mod-els of human laryngopharyngeal carcinoma FaDu cell line were established and randomly divided into normal sa-line group,sodium carboxymethyl cellulose(CMC-Na)group,cisplatin group,curcumin group and cisplatin com-bined with curcumin group(combined group).Tumor size before and after administration was compared,the tu-mor inhibition rate was calculated,CEUS and quantitative analysis were conducted to obtain blood perfusion pa-rameters,including peak intensity(PI),area under the curve(AUC),and time to peak(TTP).The tumor microves-sel density(MVD)was detected by immunohistochemical staining,and the correlation between CEUS quantita-tive parameters and MVD and tumor volume was analyzed by Pearson correlation analysis.Results:The tumor volume in the cisplatin group,curcumin group and combined group was smaller than that in the normal saline group and CMC-Na group(P＜0.05),and the tumor volume in the combined group was the smallest(P＜0.05).The tumor inhibition rates of cisplatin group,curcumin group and combined group were 43.62％,37.28％and 65.42％,respectively.The CEUS quantitative parameters PI and AUC in the cisplatin group,curcumin group and combined group were lower than those in the normal saline group and CMC-Na group(P＜0.05).MVD in the cis-platin group,curcumin group and combined group was lower than that in the normal saline group and CMC-Na group(P＜0.05),and MVD in the combined group was the lowest(P＜0.05).MVD was positively correlated with PI,AUC and tumor volume(r=0.937,0.931,0.812,all P＜0.05).PI and AUC were positively correlated with tumor volume(r=0.807,0.795,P＜0.05).Conclusion:Cisplatin combined with curcumin can enhance the growth inhibition of human laryngopharyngeal carcinoma xenografts in nude mice.CEUS quantitative parame-ters PI and AUC can effectively reflect tumor micro-angiogenesis and evaluate the chemotherapy efficacy of la-ryngopharyngeal carcinoma xenografts.
基金项目
国家自然科学基金资助项目(82160339)
国家自然科学基金资助项目(81660292)
广西自然科学基金资助项目(2024GXNSFAA010381)
广西自然科学基金资助项目(2020GXNSFAA259014)
维普
万方数据