Exploring the mechanism of matrine on lung inflammation in mice infected with respiratory syncytial virus by regulating PI3K/AKT signaling pathway
Objective:To explore the protective mechanism of matrine against respiratory syncytial virus(RSV)infection in mice based on the PI3K/AKT signaling pathway.Methods:The model of C57BL/6J mice infected with RSV virus by suspension nasal drops was established.The experiment was divided into three groups:control group,RSV group,RSV+matrine group,with 10 mice in each group.Pathological changes in mouse lung tissue were observed using hematoxylin-eosin staining(HE)and Masson staining.The levels of inflammatory factors tu-mor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6)in pulmonary alveolar lavage fluid of mice were determined by enzyme-linked immunosorbent assay(ELISA).Western blotting was performed to detect the expression of TNF-α,IL-1β,IL-6,Bax,Bcl-2,Caspase-3 and key proteins in the PI3K/AKT signaling pathway in lung tissue,and immunohistochemistry was used to detect the expression of Bax,Caspase-3,PI3K and AKT in lung tissue.Results:Compared with the control group,mice in the RSV group exhibited significant pulmonary edema,chromosomal aggregation in lung tissue cells,nuclear membrane pyknosis,marked symptoms of lung lesions,increased levels of TNF-α,IL-1β and IL-6 in lung tissue,increased expression levels of Bax and Caspase-3,and decreased expression levels of Bcl-2,P-PI3K and P-Akt(all P＜0.05).Compared with the RSV group,mice in the RSV+matrine group showed significantly alleviated pulmonary edema and pulmonary disease symptoms,significantly decreased expression levels of TNF-α,IL-1β,IL-6,Bax and Caspase-3 in lung tissue,and significantly increased expression levels of Bcl-2,P-PI3K and P-Akt(all P＜0.05).Conclusion:Matrine can inhibit the RSV-induced lung inflammation in mice and promote lung cell apoptosis,and its mechanism may be related to the activation of PI3K/AKT signaling pathway.
respiratory syncytial virusmatrinePI3K/AKT signal pathway
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维普
