Sulodexide alleviates high glucose-induced cardiac microvascular endothelial cell injury in rat by activating the AMPK/SIRT1 pathway
Objective:To study the effect and mechanism of sulodexide(SDX)on the oxidative stress and apop-tosis of cardiac microvascular endothelial cells(CMEC)in rats under high glucose(HG).Methods:CMEC of rats were isolated and cultured,and randomly divided into control group,HG group and HG+SDX group.Cell survival rates were measured by cell counting kit-8(CCK-8)assay.DCFH-DA was used as a fluorescence probe to determine the fluorescence intensity of DCF in each group to determine the level of intracellular reactive oxy-gen species(ROS).The activity of superoxide dismutase(SOD)and the content of malondialdehyde(MDA)in the supernatant were compared.The apoptosis rate was detected by flow cytometry and TUNEL assay.The ex-pression of AMPK,phosphorylated(p-)AMPK and SIRT1 proteins was detected by western blotting.Results:Compared with the control group,the HG group showed decreased cell survival rate,increased cell supernatant level of MDA,reduced SOD activity,elevated ROS level,increased apoptosis rate,and decreased expression of p-AMPK、SIRT1 proteins(all P＜0.05).Compared with the HG group,the survival rate of CMEC cells in the HG+SDX was significantly increased,the production of MDA and ROS was decreased,the activity of SOD was in-creased,the apoptosis rate of CMEC was decreased,and the expression of p-AMPK and SIRT1 proteins was sig-nificantly increased(all P＜0.05).Conclusion:SDX may alleviate HG-induced oxidative stress and apoptosis of CMEC by activating AMPK/SIRT1 signaling pathway,thus playing a cardiovascular protective role.
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维普
