Isolation and identification of polyketides from the endophytic fungus Diaporthe pseudomagif-erae derived from Acanthus ilicifolius L.and studies on their anti-oxidant and anti-inflamma-tory activities in vitro
Isolation and identification of polyketides from the endophytic fungus Diaporthe pseudomagif-erae derived from Acanthus ilicifolius L.and studies on their anti-oxidant and anti-inflamma-tory activities in vitro
Objective:To investigate the isolation and purification of the polyketide compounds and their anti-ox-idant and anti-inflammatory activities in the endophytic fungus Diaporthe pseudomagiferae from Acanthus ilicifo-lius L.Methods:The compounds were separated and purified by column chromatography on silica gel,Sephadex LH-20,and high-performance liquid chromatography,and the structures of compounds were analyzed by combin-ing with comprehensive spectral techniques such as high resolution mass spectra(MS)and nuclear magnetic reso-nance(NMR)spectroscopy.The anti-inflammatory and anti-oxidant activities of some pure compounds were evaluated.Results:A total of seven polyketides were isolated from rice fermentation products of Diaporthe pseu-domagiferae and identified as[(8-Methyl-9-oxabicyclo[4.2.1]nona-2,4-dien-7-yl)-(5-oxo-2H-furan-2-yl)meth-yl]acetate(1),2,3-Dihydromycoepoxydiene(2),Dothiorelone A(3),Dothiorelone B(4),Cytosporone B(5),5,6-dihydroxy-2,3,6-trimethylcyclohex-2-enone(6),3-(4-Methylphenoxy)propanoic acid(7).The free radical scav-enging experiment of 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)showed that compounds 1-5 did not exhib-it anti-oxidant activity.The results of enzymed-linked immunosorbent assay(ELISA)showed that compounds 1-5 had inhibitory effects on interleukin-6(IL-6)release from RAW264.7 cells induced by lipopolysaccharide(LPS).Conclusion:Compounds 1,3,4,and 6 have been isolated from the endophytic fungus Diaporthe pseudo-magiferaefrom Acanthus ilicifolius L.for the first time.Compounds 1-5 exhibit certain anti-inflammatory activity,which lays the foundation for further exploring the anti-inflammatory mechanisms of relevant active substances.
维普
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