Curcumin alleviates myocardial inflammatory responses by inhibiting Wnt5a/β-Catennin pathway after coronary microembolization in rats
Objective:To explore the potential mechanisms of curcumin on myocardial inflammatory responses following coronary microembolization(CME)in rats.Methods:Thirty-two SD rats were randomly divided into a sham group,a CME group(model group),a CME+curcumin group(curcumin group),and a CME+curcumin+Foxy-5 group(agonist group),with 8 rats in each group.The CME model in rats was established by clamping the ascending aorta and injecting inert polystyrene microspheres into the left ventricle.Real-time fluorescent quantita-tive PCR(RT-qPCR)and western blotting(WB)were employed to detect the transcription and translation levels of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),Wnt family member 5a(Wnt5a),and β-catenin in the myocardium.Results:Compared with the sham group,rats in the model group exhibited significantly decreased cardiac function,along with significantly elevated transcription and translation levels of TNF-α,IL-1β,and Wnt5a,while β-catenin expression levels showed a decrease(all P＜0.05).Compared with the model group,rats in the curcumin group showed an increasing trend in cardiac function,with significantly decreased transcription and translation levels of TNF-α,IL-1β,and Wnt5a,and an increase in β-catenin expression levels(all P＜0.05).The agonist Foxy-5 significantly reversed the effects of curcumin on the above indexes(P＜0.05).Conclusion:Curcumin can alleviate myocardial inflammatory responses by inhibiting the Wnt5a/β-catenin pathway following CME in rats.
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