Analysis on the similarity and difference of the mechanism of Guizhi decoction and formula granules alleviating blood stasis syndrome via metabolomics
Objective:To analyze the similarity and difference of the mechanism for Guizhi decoction and its for-mula granules alleviating blood stasis syndrome(BSS)in rats using proton neclear magnetic resonance spectros-copy(1 H-NMR)metabolomics.Methods:Firstly,the chemical constituents of Guizhi decoction and its formula granules were analyzed based on liquid chromatography-mass spectrometry(LC-MS)technology.Secondly,28 SD female mice were randomly divided into control group,model group,decoction group and granule group.The model was constructed with ice water bath and subcutaneous injection of adrenaline.After the modeling was com-pleted,24 h urine of rats was collected and metabolomic analysis was performed to reveal the metabolic profile regulation mechanism of BSS rats improved by Guizhi decoction and formula granules from the metabolomic level.Results:A total of 19 and 20 chemical constituents were identified in decoction and formula granules re-spectively,and 15 chemical constituents were identified together,4 chemical constituents were identified in de-coction but not in formula granules,and 5 chemical constituents were identified in formula granules but not in de-coction.Compared with the model group,the index of whole blood viscosity was significantly regressed after drug intervention in the decoction group and the granule group,and the index of red blood cell aggregation was significantly regressed in the decoction group.The metabolomics results showed that 15 differential metabolites were screened in the decoction group compared with the model group,and that it could improve BSS by modulat-ing four pathways:glycine,serine and threonine metabolism,glyoxylate and dicarboxylate metabolism,histidine metabolism,and taurine and hypotaurine metabolism.Thirteen differential metabolites were screened in the gran-ule group compared with the model group.Guizhi formula granules could alleviate BSS by regulating three path-ways:glycine,serine and threonine metabolism,glyoxylate and dicarboxylate metabolism and histidine metabo-lism.Conclusion:Both Guizhi decoction and formula granules can improve BSS by regressing disordered differ-ential metabolites and metabolic pathways,which indicates that Guizhi formula granules have similar efficacy to the decoction.
万方数据
维普
