光激活膀胱癌焦亡的新型药物的研究
Research of the novel drug for photoactivated pyroptosis of bladder cancer
郑科杰 1郑璐璐1
作者信息
- 1. 上海理工大学 光电信息与计算机工程学院,上海 200093
- 折叠
摘要
设计了一种利用牛血清蛋白(bovine serum albumin,BSA)包裹吲哚菁绿(indocyanine green,ICG)和RG108 的纳米材料ICG/RG108@BSA.吲哚菁绿在近红外激光诱导下可以活化Caspase-3 蛋白,RG108 通过抑制DNA甲基化来上调GSDME蛋白表达,从而增强了Caspase-3 蛋白切割GSDME蛋白引起的膀胱癌细胞焦亡.ICG/RG108@BSA具有优异的生物相容性,能够被膀胱癌细胞有效吞噬.ICG/RG108@BSA在 755 nm激光激活下,会对膀胱癌细胞产生明显的杀伤效果,其中小鼠膀胱癌细胞Mb49 的存活率仅为 6.9%,人膀胱移行细胞癌细胞T24 的存活率仅为 10.7%.同时 755 nm激光激发的ICG/RG108@BSA材料也成功诱导了膀胱癌细胞焦亡,为膀胱癌的肿瘤免疫治疗提供了有利的条件.
Abstract
In this paper,a nanomaterial named ICG/RG108@BSA was designed to encapsulate indocyanine green(ICG)and RG108 using bovine serum protein(BSA).Indocyanine green activated protein Caspase-3 under near infrared laser induction and RG108 up-regulated the expression of GSDME by inhibiting DNA methylation,thus enhancing the pyroptosis of bladder cancer cells caused by Caspase-3 cleavage.ICG/RG108@BSA had excellent biocompatibility,which was effectively phagocytosed by bladder cancer cells.ICG/RG108@BSA produced significant killing effect on bladder cancer cells when activated by 755 nm laser,in which the survival rate of Mb49 was only 6.9%and the survival rate of T24 cells was only 10.7%.The 755 nm laser-excited ICG/RG108@BSA nanomaterial also successfully induced bladder cancer cell pyroptosis,providing a favorable therapeutic condition for tumor immunotherapy of bladder cancer.
关键词
吲哚菁绿/膀胱癌/去甲基化/细胞焦亡Key words
indocyanine green/bladder cancer/demethylation/pyroptosis引用本文复制引用
基金项目
上海市军民融合发展专项资金科技创新支持项目(2020-jmrhkj8)
出版年
2024
NETL
NSTL
维普
万方数据