In order to solve the problem of low reproductive efficiency of seedlings,tissue culture and rapid propagation of Helleniaspeciosa were studied.Using the stem segments of Helleniaspeciosa as explants,sterile test tube seedlings were developed through primary culture,subculture,and rooting culture.The results showed that the 1/2MS+0.5～1.0 mg/L 6-BA medium had a good effect on inducing budding,with a budding rate of 81.11%;The MS+0.5 mg/L NAA+2.0～3.0 mg/L 6-BA medium had a good proliferation effect,with a proliferation rate of 3.67 times;The MS+0.2 mg/L 6-BA and 2-4 mg/L NAA media had good rooting effects on strong seedlings,with seedlings length reaching 8.07 cm and roots number reaching 6.35.This technical system provides a new approach for the rapid propagation of Helleniaspeciosa seedlings.
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