Study on Subculture Technology of Blueberry Tissue Culture
Blueberry is a new kind of health fruit,which has been paid more and more attention in many countries.The suitable medium combination of disinfection time and different hormone concentrations for subculture of blueberry in the course of primary culture was studied by using the stem segment with axillary buds as explants with IAA,CPPU,ZT and other hormones.The results showed that the best disinfection time was 5 min with 75% alcohol and 6 min with 0.1% Hg and the survival rate reached 90% .The optimal medium for callus proliferation was WPM+TDZ 0.05 mg/L+IAA 0.1 mg/L,and the adventitious bud differentiation rate reached 100% .The optimal subculture medium was WPM+IAA 0.1 mg/L+ZT 1 mg/L,the average plant height was 2.73 cm,the growth was strong and the proliferation coefficient was 5.6.This study provides experimental basis for the rapid propagation and promotion of blueberry production,and has certain research significance.
万方数据
维普
