Study on Protoplast Preparation and Regeneration of Auricularia herimuer
Auricularia herimuer is a characteristic edible fungus of Guangxi,but for a long time,the production mode of A.herimuer is still in wild or semi-wild state,In additon to lack of excellent varieties suitable for cultivation in Guangxi,the yield of A.herimuer in Guangxi is shrinking day by day,and the original market advantage is lost.Therefore,it is very important to use protoplast technology to breed,improve the varieties and restore the vitality of the strains.In this experiment,the'GuiYun 3'was used as material,the conditions of plasma preparation and regeneration were studied,including fungus age,digestion time,concentration of lysing enzymes and regeneration medium types,in order to obtain high quality,high yield of protoplasm for subsequent A.herimuer genetic breeding,excellent traits screening and breeding new varieties to provide good experimental material.The results showed that:the suitable conditions for the preparation of A.herimuer strain were as follows:mycelium culture time of 7 d,lysozyme concentration of 3％,mannitol concentration of 0.6 mol·L-1 as infiltration stabilizing agent,enzymatic hydrolysis at 30 ℃ for 3 h,a large number of protoplasts could be obtained.The protoplasts obtained under optimized conditions were regenerated,and the best regeneration culture conditions were PTA medium.These research results will provide important experimental basis and support for the future genetic breeding,selection of good traits and breeding of new varieties.
Auricularia herimuerprotoplastpreparationprotoplast regenerationregeneration medium
万方数据
维普
