首页|罗汉果根多糖的分离纯化、结构鉴定及抗肿瘤活性研究

罗汉果根多糖的分离纯化、结构鉴定及抗肿瘤活性研究

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
采用水提醇沉法提取、DEAE-52纤维素柱粗分离罗汉果根粗多糖(CPS),纸层析结合高效液相色谱分析CPS的单糖组成；同时研究CPS对小鼠H22皮下种植性肿瘤的影响.结果表明:采用DEAE-52纤维素柱粗分离得到5个多糖组分；CPS的单糖组成为:葡萄糖、阿拉伯糖、木糖,其中以葡萄糖为主；与模型对照组比较,CPS各剂量组无明显的抑制肿瘤生长作用(抑瘤率均低于50％)；与模型对照组比较,CPS各剂量组小鼠的胸腺指数显著提高,差异极显著(P＜0.01)；而脾脏指数显著降低,差异极显著(P＜0.01).
Studies on isolation、purification、structural identification and its antitumor activity of polysaccharides from Momordica grosvenori swingle’s root
Polysaccharide(CPS)of Momordica grosvenori swingle's roots was extracted by water extracting-alcohol precipitating method,and then was pufified by DEAE-52 cellulose. Their compositions were analyzed by paper chro-matography and high performance liquid chromatography(HPLC). And the effect of CPS on H22 subcutaneous planting of mice was studied. Results:CPS was isolated into five fractionates by DEAE-52 cellulose column chroma-tography,its simple sugars composition included glucose, arabillose and xylose. Compared with model group, each dose group had no significant functions in suppressing tumor growth(inhibition rate＜50%) ;Besides,Compared with model group,each dose group had improved influence on the index of thymus(P＜0. 01) ,but had reduced influence on the index of spleen(P＜0. 01).

Momordica grosvenori swingle's rootpolysaccharidesisolation and purificationstructural identificationantitumor

颜小捷、卢凤来、陈换莹、李典鹏

展开 >

广西植物功能物质研究与利用重点实验室(广西植物研究所),广西桂林541006

广西师范大学生命科学学院,广西桂林541004

罗汉果根 多糖 分离纯化 单糖组成分析 抗肿瘤

中国科学院"西部之光"人才培养计划专项药用资源化学与药物分子工程教育部重点实验室资助广西植物研究所基本业务费

科发人教字[2009]24号桂科能07109001-13桂科植1003

2012

10.3969/j.issn.1000-3142.2012.01.027

广西植物
广西壮族自治区广西植物研究所,中国科学院广西植物研究所,广西植物学会

广西植物

CSTPCDCSCD北大核心
影响因子：0.719
ISSN：1000-3142
年,卷(期)：2012.32(1)
  • 11
  • 10