Tissue culture propagation technology of Aquilaria malaccensis
Using the young shoots as explants,the methods including sterilization,induction,propagation,rooting and planting of the Aquilaria malaccensis were studied.It was indicated that the best method to sterilize was 4-5 min as 0.1% HgCl2 ;1/2 MS+6-BA 0.2 mg??L-1 +NAA 0.1 mg??L-1 +sugar 30 g??L-1 + agar 5.8 g??L-1 as the ef-fective medium for adventitious shoot induction,the induction rate was 70.5%;1/2 MS+0.1 mg??L-1 6-BA+sugar 25 g??L-1 +agar 5.8 g??L-1 as the suitable propagation medium,and the coefficient was 2.9;the medium of 1/2 MS+ sugar 30 g??L-1 + agar 5.8 g??L-1 made the emblings grow strong to cut;1/2 MS+NAA 5.0+sugar 20 g??L-1+agar 6.0 g??L-1 as the rooting medium,the rooting plantlets would be transferred to the medium with no hormone after two days.The rooting rate was 83%.It was a little difficult to transplant,and the survival rate was only 65%in the medium mixed with peat soil and yellow mud (proportion was 2∶1).
Aquilaria malaccensistissue cultureexplantsgerminate regular chemical
国家科技期刊平台
NSTL
万方数据
维普
