Study on rapid propagation and germplasm conservation in vitro of five species of genus Gynostemma in Guangxi
Using stem with buds as explants,experiments were conducted to observe the effects of different concentra-tion 6-BA and NAA 0.02 mg??L-1 on induction,differentiation,proliferation of Gynostemma in Guangxi,in order to establish their rapid propagation systems.Meanwhile,different concentrations of the mineral nutrition,sucrose and plant grow inhibitors (CCC,ABA,PP333 )were researched.The results showed that the best induction medium was MS medium supplemented with 2.0 mg??L-1 6-BA and 0.02 mg??L-1 NAA.The suitable proliferation medium for G . compressum was MS+6-BA 2.0 mg??L-1 +NAA 0.02 mg??L-1 and MS medium supplemented with 1.5 mg??L-1 6-BA and 0.2 mg??L-1 NAA was best for others.The rooting medium was 1/2MS medium with 0.02 mg??L-1 NAA, accounting for 100% of rooting rate.1/2MS medium and sucrose 40 g??L-1 were the most suitable for the conserva-tion in vitro of all species.Plant grow inhibitors could slow down the grow speed effectively,and the best kind and concentration were ABA 1.0 mg??L-1 and CCC 1.0 mg??L-1 .Several species were inhibited by CCC 1.0 mg??L-1 and the survival rate was up to 94.5% after being conserved 360 d.PP333 was not suitable for all species to conserve in vitro .Vigor testing showed that the ability of propagating and rooting of the plantlets didn’t fall down.
Gynostemma pentaphyllumG .guangxienseG .compressumG .laxumG .longipestissues cultureconservation in vitro
国家科技期刊平台
NSTL
万方数据
维普
