Different fertilization gradients affect to the soil total nutrient,available nutrients,pH,water content,organ-ic carbon and soil urease activity in alpine meadow in Maqu area were studied.To reveal the alpine meadow soil nutri-ent and enzyme activity in response to fertilization gradients.The results were as follows:(1)With the increase of fer-tilization gradients,soil pH tended to reduce obviously,fertilizing caused soil acidification in grassland ecosystem,total phosphorus and rapid available phosphorus increased obviously as the increase of fertilization gradients;(2)Soil total nitrogen,organic carbon and urease activity changed as a single peak curve as the increase of fertilization gradients.It was higher in the fertilizer gradients of 30 or 60 g??m-2 ,and the soil resources gradually reduced in the fertilizer gradi-ent of 90 g??m-2 ;(3)Seasonal variation had a certain influence on soil nutrients,total nitrogen and total phosphorus content were higher in September,The available nitrogen content was generally lower in September,available phos-phorus was lower in May;(4)The influence of fertilizer application on soil nutrients was not simple linear positive correlation.The fertilization gradients of 30-60 g??m-2 could be used as the best fertilizer levels in alpine meadow. Soil organic carbon and urease activity could be used as the important indicators to measuring soil fertilizer and soil quality in fertilization gradients.High rate of fertilization (≥90 g??m-2 )can be regarded as the threshold to effect the structure and function in alpine meadow,soil nutrient and soil enzyme activity.