Effects of different species of inorganic salt on the chlorogenic acid content and the growth of cell in suspension cultures of Lonicera macranthoides Hand.-Mazz. “Yulei 1”
In order to improve the content of chlorogenic acid in cell suspension cultures of Lonicera macranthoides Hand.-Mazz. “Yulei 1”, this paper discussed that the different species of inorganic salt had many effects on the chloro-genic acid content and growth of cell in suspension cultures of L. macranthoides Hand.-Mazz.“Yulei 1”. We weighed the biomass of the L. macranthoides Hand.-Mazz. “Yulei 1” cells and analyzed the content of chlrogenic acid by HPLC through the additions of different concentrations of inorganic salt in cell suspension cultures of L. macranthoides Hand.-Mazz. “Yulei 1”. The result showed that the optimal NO3-/NH4+ratio was consisted with NO3-/NH4+( nitrate nitrogen/ammonium nitrogen) ratio in B5 medium. In other words, the suspension culture would be benefitial for the accumulation of chlorogenicacid and growth of cell when NO3-/NH4+(nitrate nitrogen/ ammonium nitrogen) ratio was 13 ∶ 1. When single N source was added, the biomass of callus reached the maximum (19.26 g?L-1) in 3.5 g?L-1KNO3. However, when KNO3 concentration was at relatively low levels (0.5 and 1.5 g?L-1), high chlorogenic acid production was accu-mulated. The two studies of NO3-had some differences from the control concentration (2.5 g?L-1). What’s more, while (NH4)2SO4 concentration (0.268 g?L-1) exceeded control concentration (0.134 g?L-1), the highest growth of bio-mass of the L. macranthoides Hand.-Mazz. “Yulei 1” and the high chlorogenic acid production were observed. In the same way, different species and different concentrations of P, Ca and Mg might have different impacts on the chlorogenic acid content and growth of cell. The result showed that when the highest growth of biomass and the high chlorogenic acid production were accumulated, when NaH2 PO4?2H2 O concentration was 0.10 g?L-1. In addition, when the highest growth of biomass and the high chlorogenic acid production were accumulated, when CaCl2 concentration was 0.20 g?L-1 . So far as Mg2+was concerned, a low MgSO4?7H2 O concentration could promote the growth of cell, while excessive MgSO4?7H2 O concentration would promote the accumulation of chlorogenic acid. Taking the biomass growth and chloro-genic acid production into consideration, the optimal concentration of Mg was moderate. These results had some differ-ences from the control. Our study demonstrated that inorganic salt concentration had some differences from inorganic salt concentration of B5 in suspension cultures of L. macranthoides Hand.-Mazz.“Yulei 1”. This study suggested that the op-timized conditions could improve biomass accumulation and chlorogenic acid production through the addition of moderate concentration of inorganic salt in cell suspension cultures of L. macranthoides Hand.-Mazz.“Yulei 1”. These results lays a foundation for large-scale production of chlorogenic acid by using the cell suspension cultures of L. macranthoides Hand.-Mazz. “Yulei 1”. This study also has practical significance to large-scale productions of chlorogenic acid.
Lonicera macranthoides Hand.-Mazz.“Yulei 1”suspension culturechlorogenic acidHPLCinorganic salt
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