猕猴桃是一类不耐涝的植物,为了解决猕猴桃的涝害问题,吉首大学近年来尝试用‘LD-1’作为猕猴桃的耐涝性砧木。该研究以米良一号实生苗砧米良一号猕猴桃和‘LD-1’砧米良一号猕猴桃为材料,观测根系淹水后叶片可溶性糖含量、丙二醛含量、叶绿素含量、可溶性蛋白质含量、SOD 活性和形态的变化规律,探讨了‘LD-1’砧米良一号猕猴桃的耐涝性。结果表明：(1)米良一号实生苗砧米良一号猕猴桃根系淹水后,可溶性糖含量6 d 后极显著(P<0.01)升高；丙二醛含量8 d 后显著(P<0.05)升高；叶绿素含量和可溶性蛋白质含量没有显著变化；SOD 活性6 d 后显著(P<0.05)升高,8 d 后不再有显著变化；叶片2 d 后出现轻度萎蔫,10 d后全部枯死。(2)‘LD-1’砧米良一号猕猴桃根系淹水后,可溶性糖的含量4 d 后显著(P<0.05)升高；丙二醛含量、叶绿素含量、可溶性蛋白质含量和 SOD 活性无显著变化；叶片10 d 后未出现显著的萎蔫和枯死现象,30 d 后仍正常。这说明米良一号实生苗砧米良一号猕猴桃耐涝性较弱,‘LD-1’砧米良一号猕猴桃耐涝性较强,‘LD-1’作砧木显著增强了米良一号猕猴桃的耐涝性。该研究结果为‘LD-1’在米良一号猕猴桃栽培上的应用提供了依据。
Submergence tolerance of ‘LD-1’ stock-graftedActinidia deliciosa Miliang 1
Actinidia deliciosa Miliang 1, cultivated by a research team of Jishou University, Hunan Province, is a fruit tree with high output and high nutrition value but poor flood resistance, so the research on water stress is of great impor-tance for its promotion. Some professors attempted to use Actinidia ‘LD-1’ as rootstocks to improve the submergence tol-erance of A. deliciosa Miliang 1 in recent years. But in order to further reveal the submergence tolerance of ‘LD-1’ stock-grafted A. deliciosa Miliang 1 from the physiological perspective, we selected the Miliang 1 seedling stock-grafted A. deliciosa Miliang 1 and the ‘LD-1’ stock-grafted A. deliciosa Miliang 1 as experimental materials, which were trans-planted to flowerpots outside the laboratory at Jishou University in December, 2013. When the condition permitted, we treated the roots of those two materials with flood and non-flood group respectively in July, 2014. And in each group, we had 3 fruit trees for experiment. Besides recording morphological change in leaves of two experimental materials after 0, 2, 4, 6, 8 and 10 d waterlogging (both flood and non-flood group), we also tested other 5 physiological indices———sol-uble sugar content by anthrone colorimetry, malondialdhe (MDA) by thiobarbituric acid (TBA) spectrophotometric, chlorophyll content by spectrophotometry, soluble protein content by coomassie brilliant blue (CBB) G-250 and superox-ide dismutase (SOD) activity by nitroblue tetrazolium. The results were as follows: (1) When the roots of Miliang 1 seedling stock-grafted A. deliciosa Miliang 1 waterlogged, soluble sugar content in leaves of those flooded tree increased very significantly(P<0.01)6 d later compared with those non-flooded group; the content of malondialdehyde (MDA) al-so had a significant increase(P<0.05) after 8 d; and there was no significant change in chlorophyll content and soluble protein content; the activity of superoxide dismutase (SOD)increased significantly(P<0.05) 6 d later while it had no significant change after 8 d; otherwise, after 2 d waterlogging, we observed there were a small amount of leaves wilting and all leaves died after 10 d. (2) However, the damage to ‘LD-1’ stock-grafted Actinidia deliciosa Miliang 1 appeared weak. Except the content of soluble sugar increased significantly(P<0.05) 4 d later, the content of malondialdehyde (MDA), the content of chlorophyll,the content of soluble protein and the activity of superoxide dismutase (SOD)had no significant change; in addition, the leaves of ‘LD-1’ stock-grafted A. deliciosa Miliang 1 did not wilt or die 10 d later, it also seemed normal after 30 d water stress. The results, on the one hand, obviously showed that the flooding resistance of ‘LD-1’ stock-grafted A. deliciosa Miliang 1 was higher than that of Miliang 1 seedling stock-grafted A. deliciosa Mili-ang 1; the submergence tolerance of A. deliciosa Miliang 1 was enhanced significantly by Actinidia ‘LD-1’ stock. On the other hand, this research also provides a theoretical basis for the usage of ‘ LD-1’ rootstocks on A. deliciosa Miliang 1.
国家科技期刊平台
NSTL
万方数据
维普
