Effects of volatile allelochemicals from Chenopodium ambrosioides on the stoma guard cells in leaf epidermis of Vicia faba
Chenopodium ambrosioides, an aromatic herbnative to tropical Central and South America, has become a major invasive plant in China. Allelopathy plays a crictical role in the successful invasion of C. ambrosioides, and has an allelopathic stress on neighbor plants. To explore the allelopathy mechanism, the effects of volatile oil from C. am-brosioides,α-terpinene and cymene on guard cell viabilities and nucleus morphology in Vicia faba leaves were investi-gated using epidermal strips bioassay, AO/EB fluorescent staining and conventional Feulgen staining. Epidermal strips of leaves were incubated in 2-( N-morpholino) ethanesulfonic acid ( MES) buffer containing volatile oil, α-terpinene and cymene for 30 min at 25℃ in illumination incubator. The results showed that volatile oil,α-terpinene and cymene resulted in the decrease of viabilities and increase of abnormal nuclei rates in guard cell of V. faba leaves. The cytotox-icity on guard cells decreased successively from volatile oil toα-terpinene to cymene. The apoptosis features including nuclear pyknotic, malposition, stretch and degradation were observed in guard cell under the treatments of volatile oil,α-terpinene and cymene. However, the guard cell survival rates increased when strips were exposed to volatile oil,α-terpinene and cymene combined with different concentrations of a caspase inhibitor Z-VAD-FMK. These results sugges-ted that the volatile allelochemicals from Chenopodium ambrosioides induced guard cell caspase-dependent apoptosis in Vicia faba leaves.
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维普
