Inheritance analysis and discovery of chloroplast paternal inheritance in interspecific crossing of Glycyrrhiza
The goal of this paper was to have a better understanding of chloroplast inheritance patterns and identify the hybrid parent in Glycyrrhiza. A total of 68 individuals of Glycyrrhiza were selected including 34 parental species, G. ura-lensis, G. glabra, G. inflata and 34 artificial interspecific crosses, G. uralensis♀× G. glabra♂, G. glabra♀× G. ura-lensis♂, G. uralensis♀ × G. inflata♂and G. inflata♀× G. uralensis♂. Thirty-four artificial interspecific crosses were performed and the sequence polymorphisms of chloroplast DNA ( cpDNA) and internal transcribed spacer ( ITS) of nu-clear gene of parents and progenies were studied. Sequence analysis showed that ITS sequences of parents and progenies had the same length of 614 bp and altogether four variable sites, with three genotypes. Two different ITS sequence copies at four variable sites were found in each individual of F1 hybrids, one from the paternal and the other from the female parent, about which the detection ratio was 100%. It suggested that 34 artificial interspecific crosses produced successful F1 hybrids in this research and genetic trait of ITS could infer parents of Glycyrrhiza. Sequence analysis showed that the cpDNA rbcL, matK and trnH-psbA sequences of parents and artificial interspecific crosses had the same length, within a total of four variable sites and six genotypes. Two different cpDNA haplotypes were found in Glycyrrhiza. The cpDNA haplotypes of F1 hybrids matched those of the male parents and that ratio was up to 97.1%, indicating that the genetic trait was mainly inherited from the paternal parent. This result demonstrated that the chloroplast inheritance pattern of Glycyrrhiza was mostly paternal inheritance, which provides new insights into the use of cpDNA markers in studies of natural hybrids and genetic polymorphism in Glycyrrhiza.
国家科技期刊平台
NSTL
万方数据
维普
