Genetic diversity and fingerprints of Gleditsia sinensis germplasm resource based on SCoT
Genetic diversity of eighteen Gleditsia sinensis germplasms was analyzed based on SCoT markers, the cluster analysis on eighteen G. sinensis germplasms was carried out by UPGMA method, and the SCoT fingerprints of eighteen G. sinensis germplasms were constructed by the polymorphic bands. The results of amplification showed that, 226 SCoT bands were obtained from fifteen primers selected from 51 SCoT primers, including 216 polymorphic bands, with a polymorphism rate of 96.61%. The average of the polymorphism information content( PIC), observed number of alleles (Na), effective number of alleles(Ne), Nei's gene diversity (H) and Shannon's information index (I) were 0.8759, 1.9649, 1.4401, 0.2726 and 0.4261, respectively. The genetic similarity coefficients of these samples were between 0.4914 and 0.9381. Therefore, it indicates that there are rich genetic diversities among these materials. The results of cluster analysis showed that, eighteen G. sinensis germplasms could be divided into three groups when the genetic similarity coefficient was 0.60. G. heterophylla formed the first group, G. melanacantha and Zaojia-T formed the second group, and the others formed the third group. DNA fingerprints of eighteen samples were constructed from eight polymorphic loci amplified by three primers, and these materials could be distinguished and identified accurately. All these results provide the important information for the identification and breeding for new cultivars of G. sinensis.
国家科技期刊平台
NSTL
万方数据
维普
