Physiological and biochemical characteristics of Alpinia oxyphylla seeds after cryopreservation
Alpinia oxyphylla was one of the four famous south medicines in China.Its seeds were the recalcitrant seeds, which could not be preserved in dried and frozen environment,therefore,the seeds of Alpinia oxyphylla could not be re?served by regular way,in low temperature and low humidity conservation. In order to solve the A. oxyphylla seeds with long?term and stability storage,we focused on seed moisture content and freezing manner,cryopreservation method opti?mization,seed vigor indexes under liquid nitrogen,and the effects of cryopreservation on the physiological and biochemi?cal characteristics. The seed vigor indexes,including germination,activity of α?amylase,SOD,POD,dehydrogenase, MDA. The results showed that the A. oxyphylla seeds with water content of 13.92%-14.38% cryopreservated by rapid freezing could obtain the optimum effect. After liquid nitrogen freezing,the germination rate of the seeds decreased sig?nificantly,but with the extension of the freezing time,the germination rate was more than 50%.There was no significant effect on the activity of α?amylase and the content of MDA in liquid nitrogen,a decrease in the dehydrogenase and POD activity of seeds after freezing,and the activity of SOD increased with the adding of freezing time.Above results indicate that freezing time have a certain influence on seeds vigor of A. oxyphylla, but it can promote some performance of seeds.Both in germination rate,and physiological and biochemical indexes,show that A.oxyphylla seeds preserved with liquid nitrogen is feasible.
cryopreservationseeds of Alpinia oxyphyllawater contentphysiological and biochemical characteristicsseed germination rate
国家科技期刊平台
NSTL
万方数据
维普
