蛇足石杉COBRA基因家族的分子生物信息学及表达分析
Molecular bioinformatics and expression analysis of the COBRA gene family in Huperzia serrata
黄玉妹 1滕建北 1涂冬萍 1梁柳观1
作者信息
- 1. 广西中医药大学 药学院,南宁 530200
- 折叠
摘要
为探明蛇足石杉COBRA基因家族成员分子生物信息学特征及组织表达规律,该文基于蛇足石杉的全长转录组数据,通过生物信息学技术对该家族成员(HsCOBRAs)的理化性质、结构域、保守基序、顺式作用元件、基因表达量等进行分析.结果表明:(1)在蛇足石杉全长转录组中共筛选出 24 个HsCOBRAs家族成员,其中酸性蛋白 9 个,稳定蛋白 11 个,疏水性蛋白 5 个,具有跨膜结构的蛋白 7 个,具有信号肽的蛋白 3个.(2)亚细胞定位在细胞壁、叶绿体、细胞核、细胞膜上.(3)结构分析发现HsCOBRAs有 7 种结构域和 6种保守基序,部分成员具有高度保守的CCVS结构.(4)HsCOBRAs具有CAAT-box、TATA-box等 45 种顺式作用元件.(5)HsCOBRA2 在叶、孢子、茎、芽胞中的表达量均最高.该研究结果可为HsCOBRAs的进一步研究及生物学功能验证等提供理论依据.
Abstract
In order to clarify the molecular bioinformatics characteristics and tissue expression patterns of the COBRA gene family members of Huperzia serrata,the physicochemical properties,domains,conserved motifs,cis-acting elements,and genes expression of the family members(HsCOBRAs)were analyzed by bioinformatics techniques,based on the full-length transcriptome data of the H.serrata.The results were as follows:(1)A total of 24 HsCOBRAs family members were screened in the full-length transcriptome of H.serrata,including 9 acidic proteins,11 stabilizing proteins,5 hydrophobic proteins,7 proteins with transmembrane structures,and 3 proteins with signal peptides.(2)Subcellular localization was found in the cell wall,chloroplast,nucleus,and cell membrane.(3)Structural analysis revealed that HsCOBRAs had 7 domains and 6 conserved motifs,and partial members had a highly conserved CCVS structure.(4)HsCOBRAs had 45 cis-acting elements such as CAAT-box and TATA-box.(5)HsCOBRA2 had the highest expressions in leaves,spores,stems and gemma.The study results can provide theoretical basis for further research and biological function verification of HsCOBRAs.
关键词
蛇足石杉/COBRA基因家族/生物信息学/全长转录组/表达分析Key words
Huperzia serrata/COBRA gene family/bioinformatics/full-length transcriptome/expression analysis引用本文复制引用
基金项目
广西自然科学基金(2022JJA140897)
广西中医药大学桂派中医药传承创新团队项目(2022B005)
广西中医药大学桂派杏林青年英才项目(2022C033)
广西中医药大学研究生教育创新计划项目(YCSY2022007)
出版年
2024
国家科技期刊平台
NSTL
万方数据