首页|蛇足石杉COBRA基因家族的分子生物信息学及表达分析

蛇足石杉COBRA基因家族的分子生物信息学及表达分析

Molecular bioinformatics and expression analysis of the COBRA gene family in Huperzia serrata

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原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
为探明蛇足石杉COBRA基因家族成员分子生物信息学特征及组织表达规律,该文基于蛇足石杉的全长转录组数据,通过生物信息学技术对该家族成员(HsCOBRAs)的理化性质、结构域、保守基序、顺式作用元件、基因表达量等进行分析.结果表明:(1)在蛇足石杉全长转录组中共筛选出 24 个HsCOBRAs家族成员,其中酸性蛋白 9 个,稳定蛋白 11 个,疏水性蛋白 5 个,具有跨膜结构的蛋白 7 个,具有信号肽的蛋白 3个.(2)亚细胞定位在细胞壁、叶绿体、细胞核、细胞膜上.(3)结构分析发现HsCOBRAs有 7 种结构域和 6种保守基序,部分成员具有高度保守的CCVS结构.(4)HsCOBRAs具有CAAT-box、TATA-box等 45 种顺式作用元件.(5)HsCOBRA2 在叶、孢子、茎、芽胞中的表达量均最高.该研究结果可为HsCOBRAs的进一步研究及生物学功能验证等提供理论依据.
In order to clarify the molecular bioinformatics characteristics and tissue expression patterns of the COBRA gene family members of Huperzia serrata,the physicochemical properties,domains,conserved motifs,cis-acting elements,and genes expression of the family members(HsCOBRAs)were analyzed by bioinformatics techniques,based on the full-length transcriptome data of the H.serrata.The results were as follows:(1)A total of 24 HsCOBRAs family members were screened in the full-length transcriptome of H.serrata,including 9 acidic proteins,11 stabilizing proteins,5 hydrophobic proteins,7 proteins with transmembrane structures,and 3 proteins with signal peptides.(2)Subcellular localization was found in the cell wall,chloroplast,nucleus,and cell membrane.(3)Structural analysis revealed that HsCOBRAs had 7 domains and 6 conserved motifs,and partial members had a highly conserved CCVS structure.(4)HsCOBRAs had 45 cis-acting elements such as CAAT-box and TATA-box.(5)HsCOBRA2 had the highest expressions in leaves,spores,stems and gemma.The study results can provide theoretical basis for further research and biological function verification of HsCOBRAs.

Huperzia serrataCOBRA gene familybioinformaticsfull-length transcriptomeexpression analysis

黄玉妹、滕建北、涂冬萍、梁柳观

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广西中医药大学 药学院,南宁 530200

蛇足石杉 COBRA基因家族 生物信息学 全长转录组 表达分析

广西自然科学基金广西中医药大学桂派中医药传承创新团队项目广西中医药大学桂派杏林青年英才项目广西中医药大学研究生教育创新计划项目

2022JJA1408972022B0052022C033YCSY2022007

2024

10.11931/guihaia.gxzw202307014

广西植物
广西壮族自治区广西植物研究所,中国科学院广西植物研究所,广西植物学会

广西植物

CSTPCD北大核心
影响因子：0.719
ISSN：1000-3142
年,卷(期)：2024.44(6)