Chemical constituents and cytotoxic activity of Ludwigia octovalvis
To study the chemical constituents of Ludwigia octovalvis,the 80%ethanol extract from L.octovalvis was isolated by silica gel,Sephadex LH-20,Flash C18 and semi-preparative liquid chromatography,and the structures of obtained compounds were identified by physicochemical properties and spectral data.The cytotoxic activity of the isolates were evaluated by MTS method.The results were as follows:(1)Twenty compounds were isolated from L.octovalvis,and their structures were identified as(-)-lyoniresinol(1),8,8′-bisdihydrosiringenin(2),5-methoxy-(-)-isolariciresinol(3),(-)-isolariciresinol(4),3,4′-di-O-methylellagic acid(5),3,3′,4′-tri-O-methylellagic acid(6),1,3,6-tri-O-galloyl-β-glucospyranose(7),corilagin(8),methyl gallate(9),ethyl gallate(10),terminaliate A(11),syringic acid(12),3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone(13),luteolin(14),kaempferol(15),5,8-dihydroxy-7-methoxyflavone(16),nobiletin(17),tangeretin(18),α-tocopherolquinone(19),5-O-(E)-p-coumaroyl quinic acid ethyl ester(20).Compounds 1-5,7,8,11,13,16-20 were isolated from Ludwigia for the first time.Compounds 6,9,10,12,15 were isolated from this plant for the first time.(2)Compound 19 showed inhibitory activity against the leukemia HL-60 with the IC50 value of 10.31 μmol·L-1.Compounds 6-8,19 showed inhibitory activity against the lung cancer cells A549 with the IC50 values of 25.82,42.05,36.94,17.54 μmol·L-1,respectively.Compounds 6,7,11,14,19 showed inhibitory activity against the liver cancer SMMC-7721 with the IC50 values of 24.24,26.35,26.51,33.34,20.44 μmol·L-1,respectively.Compounds 6,19 showed inhibitory activity against the breast cancer MDA-MB-231 with the IC50 values of 34.91,21.13 μmol·L-1,respectively.Compounds 6,7,19 showed inhibitory activity against the colon cancer SW480 with the IC50 values of 36.03,39.97,5.52 μmol·L-1,respectively.The results of this study enrich the chemical constituents of L.octovalvis and provide a basis for the development of anti-tumor activity.
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