Genome-wide identification and abiotic stress analysis of TCP transcription factors in tartary buckwheat
TCP is a plant-specific transcription factor that plays crucial roles in plant growth and development.In this study,bioinformatics methods were used to identify the complete genome of tartary buckwheat TCP family,and real-time fluorescence quantitative PCR(qRT-PCR)analysis was conducted to investigate the expression characteristics of TCP gene under drought and salt stresses.The results were as follows:(1)A total of 28 TCP family members were identified in the tartary buckwheat genome,unevenly distributed across its eight chromosomes.(2)Most tartary buckwheat TCP genes contained 1-5 exons.(3)Phylogenetic analysis classified the tartary buckwheat TCP family into five clades,with intraspecific TCP proteins mainly clustering together.(4)Collinearity analysis indicated that there were five tartary buckwheat TCP genes originated from genome-wide replication events.(5)Cis-element analysis revealed that the promoter regions of tartary buckwheat TCP genes predominantly contained two types of cis-response elements as stress response elements and hormone response elements.(6)Transcriptomic data analysis demonstrated that all tartary buckwheat TCP genes were expressed in the examined tissues.(7)qRT-PCR results indicated that the expression levels of FtTCP3,FtTCP6,FtTCP12,and FtTCP13 changed under drought stress and salt stress conditions,with FtTCP3 peaking at 6 h of drought and salt treatments,suggesting that it plays a positive regulatory role in tartary buckwheat's response to drought stress and salt stress.This study provides new insights into the evolution and function of the TCP gene family,and provides a reference for the functional exploration and utilization of the tartary buckwheat TCP gene family.
万方数据
维普
