Effects of bacterial agent application on physiological characteristics of photosynthesis and stress resistance in Dicranopteris pedata under high temperature stress
In order to investigate study the influence of microbial agent Bacillus natto on the ability of Dicranopteris pedata to tolerate high temperature stress,we determined the physiological indexes of photosynthesis and resistance to high temperature of annual D.pedata under different temperatures and fertilizer treatments.The results were as follows:(1)Both temperature and fertilizer could significantly affect the photosynthesis and high temperature resistance physiology of D.pedata(P＜0.05).(2)The net photosynthetic rate,transpiration rate,stomatal conductance,and chlorophyll content of D.pedata leaves were significantly reduced(P＜0.05)when the temperature was increased to 45 ℃.The inhibition of photosynthetic physiology of Dicranopteris pedata by high-temperature stress was an non-stomatal limiting.To defend the high temperature stress,the superoxide dismutase,peroxidase activity,proline,malondialdehyde content,and relative conductivity of D.pedata were increased significantly(P＜0.05).(3)Compared with the control group and the organic fertilizer group,the fertilizer with microbial agent audition significantly increased the net photosynthetic rate,transpiration rate,stomatal conductance,chlorophyll content,and the activities of peroxide dismutase and catalase in leaves of D.pedata,and decreased the intercellular CO2 concentration,malondialdehyde content and the relative electrical conductivity of D.pedata leaves(P＜0.05).(4)The evaluation method combining principal component analysis and membership function method was used to evaluate the high temperature resistance of D.pedata.It was found that the D.pedata scored higher in resistance to high temperature under fertilizer-added origin manure application.Overall,D.pedata has a certain degree of resistance to high temperature,and can selectively induce stress resistance physiology to adapt the high temperatures environment according to different stress environments.Bacillus natto can alleviate the photosynthesis inhibition of Dicranopteris pedata by high temperature stress,induce the increase of antioxidant enzyme activities to alleviate cell damage,reduce the pressure of osmoregulation,and effectively improved the resistance of D.pedata induced by high temperature stress.This study provide a reference for the ecological restoration and soil and water conservation in the southern red soil erosion area and similar areas.
Dicranopteris pedatahigh temperature stressBacillus nattophotosynthetic characteristicsstress resistance physiology
万方数据
维普
