Cloning and expression analysis of maize fucosyltransferase gene SPINDLY
SPINDLY(SPY)is a novel nucleocytoplasmic protein O-fucosyltransferase that regulates target protein activity or stability via O-fucosylation.Previous studies have indicated that the SPY protein regulates plant growth and development by modulating various intracellular processes in Arabidopsis thaliana.Its mediated O-fucosylation plays an important role in maintaining cell homeostasis and regulating plant growth and development,however protein O-fucosylation regulated by SPY in other plants largely remain unknown.Maize(Zea mays)is one of the most important cereals crops for supplying foods,fibers,and fuels to humans.In order to explore the function of maize fucosyltransferase gene(ZmSPY),this study first analyzed the conserved domain,amino acid sequence and physicochemical properties of ZmSPY protein by bioinformatics method,and cloned the gene from maize root tissue to construct the GFP fusion protein expression vector.The subcellular localization of ZmSPY was analyzed,and its response to different hormone treatments(GA,IAA,6BA,ABA)was determined by exogenous hormone application.The results were as follows:(1)ZmSPY proteins belonged to the TPR and SPY superfamilies,and structural analysis demonstrated that ZmSPY had TPR(Tetratricopeptide repeat)and catalytic domains.(2)Phylogenetic analysis showed that SPYs were highly conserved,and ZmSPY exhibited strong homology to SPY in Sorghum bicolor.(3)Sequence analysis showed that the CDS region of ZmSPY was 2 736 bp.Physicochemical analysis indicated that ZmSPY,which contained 911 amino acids and 33 glycosylation sites,was hydrophilic and non-secretory.Its secondary and tertiary structures were largely composed of alpha helix and random coil.(4)The subcellular localization of ZmSPY was predominantly observed in the nucleus.(5)The expression of ZmSPY was induced by phytohormones including GA,IAA,6BA and ABA,and exhibited various expression patterns.This study provides foundational information on SPY in maize,which contributes to further investigation of SPY and its effect on O-fucosylation in cereal plants.
万方数据
维普
