[Objective]To establish a method for the determination of shikimic acid,protocatechuic acid,p-hy-droxybenzoic acid and p-methoxybenzoic acid in star anise.[Methods]The star anise was determined by high perfor-mance liquid chromatography.The chromatographic column was Kromasil 100-5-C18(250 mm×4.6 mm,5 μm).The mobile phase was acetonitrile-3％phosphoric acid aqueous solution(gradient elution).The flow rate was 1.0 ml/min,the injection volume was 10μl,the detection wavelength was 254 nm,and the column temperature was 40℃.[Results]The contents of shikimic acid,protocatechuic acid,p-hydroxybenzoic acid and p-methoxybenzoic acid in star anise from 11 different producing areas in Guangxi were 6.34％～7.41％,0.28％～0.98％,0.73％～1.90％and 0.07％～0.23％,respectively.[Conclusion]The method applied in this study is simple,stable and reproducible,and can be used for the determination of active ingredients in star anise.
关键词
八角茴香/莽草酸/原儿茶酸/对羟基苯甲酸/对甲氧基苯甲酸/高效液相色谱法/含量测定
Key words
star anise/shikimic acid/protocatechuic acid/p-hydroxybenzoic acid/p-methoxybenzoic acid/high performance liquid chromatography/content determination
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