Mechanism Study on Tanshinone ⅡA Alleviating Heat Stress-Induced Testicular Cell Apoptosis in Mice Through Transcriptome Sequencing
[Objective]To study the effect of tanshinone ⅡA(TSA)on testicular impairment in mice induced by heat stress and its mechanism of action.[Methods]Sixty C57 mice were randomly divided into control group,heat stress(HS)group,and TSA-low,TSA-medium,TSA-high dose group,with 12 mice in each group.Except for those in control group,the mice were treated with a single testicular heat stress treatment to build the testicular impairment model.Start-ing from the next day of modeling,7-day intraperitoneal injection of 10 μg/g,20 μg/g,and 40 μg/g TSA DMSO solution was respectively performed in TSA-low,TSA-medium,and TSA-high dose group.Within the same period of time,the intraperitoneal injection of same volume of DMSO was performed in control group.The effect and its mechanism of TSA were evaluated by the techniques of pathology,high-throughput sequencing,bioinformatic analysis and molecular biolo-gy.[Results]Compared with control group,the diameter of testicular seminiferous tubules of mice in the HS group was significantly reduced,apoptosis in testicular tissue was significantly increased,and testosterone level was significantly decreased(P<0.05).In the TSA groups,the diameter of testicular seminiferous tubules was significantly increased,apoptosis in testicular tissue was significantly reduced,and testosterone level was significantly increased(P<0.05),compared with HS group.In high-throughput transcriptome sequencing,the key differentially expressed genes of the mice in the HS group,the control group and the TSA-medium group were mainly found in the cell apoptosis signaling pathway.The results of Western blot showed that the cell apoptosis in the HS group,which led to organic lesions of tes-ticular tissue and testicular secretory function impairment,was induced by the up-regulating the protein expression in the apoptosis pathway.TSA treatment can inhibit the expression of proteins in the apoptosis pathway and alleviate the testicular impairment caused by heat stress.[Conclusion]TSA can alleviate the mice testicular impairment induced by heat stress,and its mechanism may relate to inhibiting cell apoptosis.
tanshinone ⅡAheat stresstesticular impairmentapoptosisexperimental research
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