Effects of Yishen Tongxie Formula on the Expression of IL-2,IL-10,TGF-β and IFN-γ in Rats with Diarrhea-type Irritable Bowel Syndrome
[Objective]To study the effects of Yishen Tongxie formula on the expression levels of IL-2,IL-10,TGF-β and IFN-γ in the small intestine of rats with diarrheal irritable bowel syndrome.[Methods]Twenty-four wistar rats were divided into normal group,model group,Otilonium bromide group and Yishen Tongxie formula group by ran-dom number table method,with 6 rats in each group.By using 4%acetic acid enemas combined with chronic psycholog-ical stress,the diarrhea-type irritable bowel syndrome model was built in all groups except the normal group.In the Oti-lonium bromide group and Yishen Tongxie formula group,the related drugs were administered for 14 days after the mod-el was built.The general condition of the rats was observed before execution.HE staining was used to observe the patho-logical changes of small intestine,and RT-PCR and Western blot were used to measure the mRNA and protein expres-sion levels of IL-2,IL-10,TGF-β and IFN-γ in small intestine.[Results]Compared with the normal group,obvious focal lymphocyte infiltration in the small intestine of the rats in the model group was observed,and the mRNA and pro-tein expression levels of IL-2,IL-10,TGF-β and IFN-γ in the small intestine of the rats in the model group were sig-nificantly increased(P<0.01).Compared with the model group,no obvious inflammatory infiltration in small intestinal tissues was observed in Otilonium bromide group and Yishen Tongxie formula group,and the mRNA and protein expres-sion levels of IL-2,IL-10,TGF-β and IFN-γ were significantly decreased in Otilonium bromide group and Yishen Tongxie formula group(P<0.05 or P<0.01).Compared with Otilonium bromide group,abundant intestinal glands in the lamina propria of small intestine and no obvious inflammatory cell infiltration were observed in Yishen Tongxie formula group,and the mRNA and protein expressions of IL-2,IL-10,TGF-β and IFN-γ were lower than those of Otilonium bromide group,with no statistical significance(P>0.05).[Conclusion]Yishen Tongxie formula may alleviate the dam-age of intestinal inflammation and play a role in the treatment of diarrhea-type irritable bowel by reducing the expres-sion of IL-2,IL-10,TGF-β,and IFN-γ.
Yishen Tongxie formuladiarrheal irritable bowel syndromeintestinal inflammatory responsesIL-2IL-10TGF-βIFN-γexperimental study
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