Effect of Qingzao Yangfei Decoction on Th1/Th2 immune regulation in NOD mice with Sjogren's syndrome
Objective:To study the intervention effect of Qingzao Yangfei Decoction on Th1/Th2 cell immune im-balance and the expression of related cytokines in Non-obese diabetic(NOD)mice with Sjogren's syndrome,and to ex-plore the mechanism of Qingzao Yangfei Decoction in the treatment of Sjogren's syndrome.Methods:A total of 100 fe-male NOD mice with Sjogren's syndrome were randomly divided into model group,hydroxychloroquine sulfate group and Qingzao Yangfei Decoction low,medium and high dose groups,with 20 mice in each group.The mice in the model group were given 0.9%sodium chloride injection by intragastric administration.The hydroxychloroquine sulfate group was given hydroxychloroquine sulfate solution(60 mg/kg)by intragastric administration.The low,medium and high dose groups of Qingzao Yangfei Decoction were given Qingzao Yangfei Decoction by intragastric administration at 30 g/kg,60 g/kg and 120 g/kg,respectively.The intragastric volume was 10 ml/kg.The salivary flow rate of mice was mea-sured by wet weight method at the 2nd,4th,6th and 8th week of the experiment.After 8 weeks of the experiment,blood was taken from the femoral artery to determine the levels of serum Th1-related cytokines as interleukin(IL)-2,interfer-on(IFN),tumor necrosis factor-α(TNF-α)and Th2-related cytokines(IL-4,IL-6,IL-10,IL-13)in each group.Results:From the 2nd to 6th week of the experiment,the salivary flow rate of mice in each administration group was higher than that in the model group(P<0.05).At the 6th week of the experiment,the salivary flow rate of mice in the high-dose Qingzao Yangfei Decoction group was higher than that in the hydroxychloroquine sulfate group(P<0.05).At the 8th week of the experiment,the salivary flow rate of mice in the medium and high dose groups of Qingzao Yangfei Decoction was higher than that in the hydroxychloroquine sulfate group(P<0.05).Compared with the model group,the levels of serum Th1 cytokines(IFN,TNF-α,IL-2)and Th2-related cytokines(IL-4,IL-6,IL-10,IL-13)in each treat-ment group were significantly decreased(P<0.05),and the levels of IFN,TNF-α,IL-2 and IL-4,IL-10,IL-13 in the high-dose group of Qingzao Yangfei Decoction were lower than those in the hydroxychloroquine sulfate group(P<0.05).Conclusion:Qingzao Yangfei Decoction can increase the salivary flow rate of NOD mice with Sjogren's syndrome and reduce the level of Th1/Th2 related cytokines in serum.It may play a therapeutic role by regulating Th1/Th2 immune balance and inhibiting the proinflammatory effect of cytokines.
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