基于NF-κB信号通路研究鸡娃草石油醚提取物对LPS诱导RAW264.7细胞的抗炎作用

Stuy on anti-inflammatory effect of petroleum ether extract of Plumbagella micrantha on LPS-induced RAW264.7 cells through NF-κB signaling pathway

扫码查看

原文链接

维普
万方数据

中文摘要：目的:观察鸡娃草石油醚提取物(Pm-PE)通过核因子κB(NF-κB)信号通路对脂多糖(LPS)诱导RAW264.7细胞的抗炎作用.方法:以不同浓度Pm-PE作用于RAW264.7细胞24 h,分析在LPS刺激下药物对细胞形态变化的影响;采用CCK-8法检测不同浓度的Pm-PE对RAW264.7细胞活性的影响;以一氧化氮(NO)试剂盒法检测致RAW264.7炎症细胞的NO释放量;以酶联免疫吸附法(ELISA)检测肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)和白介素-10(IL-10)的分泌;以逆转录聚合酶链反应法(RT-PCR)检测促炎因子诱导型一氧化氮合酶(iNOS)、TNF-α、IL-6和抗炎因子IL-10的mRNA表达情况;以免疫印迹法(Western blot)检测细胞NF-κB p65、IκB-α蛋白的表达.结果:与LPS模型组比较,Pm-PE不同剂量组能剂量依赖性显著降低NO、TNF-α、IL-6的分泌水平和下调iNOS、TNF-α、IL-6 mRNA的表达,同时调节抗炎因子IL-10的表达;Pm-PE中、高剂量组能显著性抑制NF-κB p-p65和p-IκB-α蛋白的相对表达水平.结论:鸡娃草石油醚提取物可通过NF-κB信号通路减少LPS诱导的RAW264.7细胞炎症相关因子的释放,从而发挥抗炎作用.

外文摘要：Objective:To observe the anti-inflammatory effect of petroleum ether extract of Plumbagella micrantha(Pm-PE)on lipopolysaccharide(LPS)-induced RAW264.7 cells through nuclear factor-κB(NF-κB)signaling path-way.Methods:RAW264.7 cells were treated with different concentrations of Pm-PE for 24 h,and the effects of drugs on cell morphology under LPS stimulation were analyzed.The effect of different concentrations of Pm-PE on the activi-ty of RAW264.7 cells was detected by Cell Counting Kit-8(CCK-8).The NO release of RAW264.7 inflammatory cells was detected by nitric oxide(NO)kit method.The secretion of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-10(IL-10)was detected by enzyme-linked immunosorbent assay(ELISA).Reverse transcription poly-merase chain reaction(RT-PCR)was used to detect pro-inflammatory factors.The mRNA expression of inducible ni-tric oxide synthase(iNOS),TNF-α,IL-6 and anti-inflammatory factor IL-10 were detected by RT-PCR.The expres-sion of NF-κB p65 and IκB-α protein was detected by Western blot.Results:Compared with the LPS model group,Pm-PE at different doses could significantly reduce the dose dependence.NO,TNF-α,IL-6 secretion and iNOS,TNF-α,IL-6 mRNA expression,while regulating the expression of anti-inflammatory factor IL-10;the medium and high dose groups of Pm-PE could significantly inhibit the relative expression levels of NF-κBp-p65 and p-IκB-α protein.Conclusion:The petroleum ether extract of Plumbagella micrantha can reduce the release of inflammation-related fac-tors in RAW264.7 cells induced by LPS through NF-κB signaling pathway,thus exerting anti-inflammatory effects.

外文关键词：

Plumbagella micranthaPetroleum ether extractMouse macrophage cell lineLipopolysaccharideAnti-inflammatoryNF-κB signaling pathway

作者：

莫婷、何婷、杨丽娟、杨森、康菊英、马静、赵芳芳、火明才、卢海瑞

展开 >

作者单位：

宁夏回族医药研究所,宁夏银川 750021

关键词：

鸡娃草石油醚提取物小鼠巨噬细胞系脂多糖抗炎 NF-κB信号通路

基金：

宁夏自然科学基金项目宁夏回族自治区卫生健康系统科研课题宁夏中药资源保障能力提升项目

项目编号：

2023AAC037182021-NW-059宁卫办发[2023]34号

出版年：

2024

广西中医药

广西中医学院中国中医药学会广西分会

广西中医药

影响因子：0.611

ISSN：1003-0719

年,卷(期)：2024.47(2)

参考文献量17