Objective:To observe the anti-inflammatory effect of petroleum ether extract of Plumbagella micrantha(Pm-PE)on lipopolysaccharide(LPS)-induced RAW264.7 cells through nuclear factor-κB(NF-κB)signaling path-way.Methods:RAW264.7 cells were treated with different concentrations of Pm-PE for 24 h,and the effects of drugs on cell morphology under LPS stimulation were analyzed.The effect of different concentrations of Pm-PE on the activi-ty of RAW264.7 cells was detected by Cell Counting Kit-8(CCK-8).The NO release of RAW264.7 inflammatory cells was detected by nitric oxide(NO)kit method.The secretion of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-10(IL-10)was detected by enzyme-linked immunosorbent assay(ELISA).Reverse transcription poly-merase chain reaction(RT-PCR)was used to detect pro-inflammatory factors.The mRNA expression of inducible ni-tric oxide synthase(iNOS),TNF-α,IL-6 and anti-inflammatory factor IL-10 were detected by RT-PCR.The expres-sion of NF-κB p65 and IκB-α protein was detected by Western blot.Results:Compared with the LPS model group,Pm-PE at different doses could significantly reduce the dose dependence.NO,TNF-α,IL-6 secretion and iNOS,TNF-α,IL-6 mRNA expression,while regulating the expression of anti-inflammatory factor IL-10;the medium and high dose groups of Pm-PE could significantly inhibit the relative expression levels of NF-κBp-p65 and p-IκB-α protein.Conclusion:The petroleum ether extract of Plumbagella micrantha can reduce the release of inflammation-related fac-tors in RAW264.7 cells induced by LPS through NF-κB signaling pathway,thus exerting anti-inflammatory effects.
维普
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