Effects of salidroside on mitochondrial function in the brain and hippocampal cell proliferation in AD model mice
Objective:To explore the effects of salidroside on mitochondrial function in the brain tissue and hip-pocampal dentate gyrus cell proliferation of AD model mice induced by D-galactose combined with sodium nitrite.Methods:A total of 45 mice were randomly divided into the normal group,model group,and salidroside group,with 15 mice in each group.Mice in the salidroside and model groups received daily intraperitoneal injection of sodium nitrite(50 mg/kg)and subcutaneous injection of D-galactose(150 mg/kg)for 60 days,while the normal group received equiv-alent volumes of saline via intraperitoneal and subcutaneous injections.During the modeling period,mice in the salidro-side group administered subcutaneous injections of salidroside(40 mg/kg),while mice in the normal and model groups received saline injections of equivalent volumes.After administration,mitochondrial function in the brain tissue and proliferation levels of hippocampal dentate gyrus neural stem cells in mice were evaluated.Mitochondrial membrane po-tential was detected using JC-1 fluorescent probe,respiratory control ratio(RCR)of mitochondria was determined us-ing Clark-type oxygen electrode method,adenosine triphosphate(ATP)content was measured using the luciferase bio-luminescence method,and proliferation cells were labeled with BrdU and hippocampal stem cell proliferation levels were assessed using BrdU immunohistochemistry technique.Results:Compared to the normal group,the model group of mice exhibited significantly reduced levels of mitochondrial membrane potential,RCR,and ATP contents,as well as a noticeable decrease in BrdU-positive cell counts,with significant differences(P<0.05).When compared to the model group,the salidroside group of mice showed remarkable increased levels of mitochondrial membrane potential,RCR,and ATP contents,along with a significant increase in BrdU-positive cell counts,demonstrating significant differences(P<0.05).Conclusion:Salidroside exhibits a certain protective effect on mitochondrial function in the brain tissue of AD model mice,while also enhancing the proliferation levels of granule cells in the subgranular zone of the hippocam-pal dentate gyrus,indicating its potential role in promoting neurogenesis.
salidrosidesenile dementiamitochondrial functionhippocampal cellneurogenesisexperimental study
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