Study on the protective effect of salidroside on oxidative stress and cognitive impairment in AD rats
Objective:To study the protective effect of intraperitoneal injection of salidroside on oxidative stress and cognitive impairment in rats with Alzheimer's disease(AD)induced by bilateral intracerebroventricular injection of streptozotocin(STZ).Methods:A total of 40 adult male SD rats were randomly divided into 4 groups:normal control group,model group,salidroside high dose group,salidroside low dose group,with10 rats in each group.In addition to the normal control group,the other groups were injected with STZ into the bilateral ventricles twice to prepare the AD animal model,and the normal control group was injected with artificial cerebrospinal fluid into the bilateral ventricles.After modeling,the high-dose(50 mg/kg)and low-dose(25 mg/kg)salidroside groups were intraperitoneally injected with salidroside for 3 weeks,and the model group and the normal control group were intraperitoneally injected with the same volume of normal saline.The changes of cognitive behavior in rats were detected by Morris water maze(MWM).The level of ROS in hippocampal homogenate was detected by reactive oxygen species(ROS)fluorescent probe 2,7-di-chlorofluorescein diacetate(DCFH-DA).The activity of total superoxide dismutase(T-SOD)in plasma and hippocam-pus was determined by hydroxylamine method.The activity of catalase(CAT)was determined by ammonium molybdate method.The activity of glutathione peroxidase(GSH-Px)was determined by colorimetry.The content of malondialde-hyde(MDA)was detected by malondialdehyde(MDA)assay kit(TBA).Results:The results of MWM positioning navi-gation experiment and spatial exploration test showed that the spatial learning and memory retention ability of rats was significantly impaired after bilateral intracerebroventricular injection of STZ.The intraperitoneal injection of salidro-side had a certain protective effect,and the high dose effect was better.Compared with the normal control group,the level of ROS in hippocampus homogenate of rats in the model group was significantly increased(P<0.05),the activities of T-SOD,CAT and GSH-Px in plasma and hippocampus were significantly decreased(P<0.05),and the level of MDA was significantly increased(P<0.05).Compared with the model group,the levels of ROS in hippocampus homogenate of rats in salidroside high and low dose groups were significantly decreased(P<0.05),the activities of T-SOD and GSH-Px in plasma and hippocampus were significantly increased(P<0.05),and the levels of MDA were significantly decreased(P<0.05),and the effect of salidroside high dose group was better than that of salidroside low dose group may be related to increasing the activities of T-SOD,CAT and GSH-Px in vivo,reducing the level of ROS in hippocam⁃ pus and alleviating oxidative damage.
SalidrosideAlzheimer's diseaseOxidative stressCognitive impairmentProtective effectExperi-mental research
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