Effects of GEI HERBA extract on learning and memory ability of mice with Alzheimer's disease and its mechanism of action
Objective:To observe the effects of GEI HERBA extract on the learning and memory ability of Al-zheimer's disease(AD)mice and study its mechanism of action.Methods:48 mice were randomly divided into 6 groups,which are normal control group,model group,positive control group and GEI HERBA low,medium and high dose groups,with 8 mice in each.Except for the normal control group,the other groups were injected with sodium ni-trite intraperitoneally and D-galactose subcutaneously to replicate AD model mice.The treatment drugs were given orally during the process of modeling.The animals in positive control group were given piracetam solution(0.8 g/kg),and those in the low-,medium-,and high-dose groups were given GEI HERBA extract solution(2.5 g/kg,5 g/kg,and 10 g/kg,respectively).The administrative volume was 20 ml/kg.The mice in the normal control group and the model group were given the same volume of normal saline.After 2 months of treatment,the learning and memory ability was de-termined by Morris water maze,the expression of nuclear factor κB(NF-κB)p65,tumor necrosis factor α(TNF-α),phosphatidylinositol 3-kinase(PI3K),and protein kinase B(Akt)in the cerebral cortex were determined by immunohis-tochemical analysis.Results:Compared with the normal control group,the escape latency of the mice in the model group was significantly prolonged(P＜0.05),the number of crossing the platform was significantly reduced(P＜0.05),and the expression of NF-κB p65,TNF-α,Akt,and PI3K in the cerebral cortex of mice was significantly increased(P＜0.05);compared with the model group,the escape latency of the mice in the positive control group and the high and me-dium dose groups was significantly shortened(P＜0.05),and the number of crossing the platform was significantly in-creased(P＜0.05);compared with the model group,the expression of NF-κB p65,TNF-α,PI3K,and Akt in the cere-bral cortex of AD mice was significantly reduced in the positive control group and the high,medium,and low dose groups(P＜0.05).Conclusion:GEI HERBA extract can improve the learning and memory ability of AD mice,and its mechanism of action may be related to the inhibition of the expression of NF-κB p65,TNF-α,PI3K,and Akt in the ce-rebral cortex.
GEI HERBAL extractAlzheimer's diseaselearning and memory abilityNF-κBPI3K/Akt signaling pathway
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