Study and deviation analysis for the determination of particle size based on nanopores Coulter method
The Coulter measurement method based on nanopores can measure particle size down to 100 nm or even smaller.In this study,the standard polystyrene microspheres and astaxanthin are used to evaluate the particle size measurement results of the Coulter method based on nanopores.As a result,the data shows that the measurement error of standard microsphere particle size is better than±10％,and the repeatability is better than 1％;for the measurement of astaxanthin,attention should be paid to the salt ion concentration of the sample's dilute solution and oscillation frequency changes of the pretreat-ment,which may cause sample damage and form large particles.These may result in the larger sample size and size distribution shifting.It should pay attention to the impact of the salt ion concentration of the diluent and sample pretreatment conditions on the measurement error when using a nanopore-based Coul-ter analyzer to measure biological products.The accuracy of the measurement results can be ensured by optimizing methods and processing techniques.
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