Purification by Microporous Resin and Antioxidant Activity in vitro of Anthocyanins from Rubus coreanus Maq
The anthocyanins from fresh fruit of Rubus coreanus Maq.was isolated and purified by AB-8 microporous res-in,and its antioxidant activity was studied.Treatment at pH 1 of the sample solution,anthocyanins concentration of the sample solution was 0.75 mg/mL,adsorption and desorption flow rates were 1mL/min and the elution solvent was 6BV of 70%ethanol,a good purification effect was achieved,and the ratio of color value of anthocyanins before and after purification is 1∶15.7.In addition,anthocyanins after purification from fresh fruit of Rubus coreanus Maq.was evaluated for its antioxidant activity.It was found that anthocyanins displayed dose-dependent reducing power,positive linear cor-relation and strong abilities to scavenge DPPH and ABTS free radicals in the concentration range investigated here.And anthocyanins possessed free radical scavenging activity against ABTS(IC50=0.6349 μg/mL)and DPPH(IC50=0.9044 μg/mL)radicals in decreasing order as well as reducing power,the inhibiting abilities were far higher than VC as posi-tive controls.These results show that anthocyanins from Rubus coreanus Maq.have potent antioxidant activity.
Rubus coreanus MaqAnthocyaninsseparation and PurificationMicroporous resinAntioxidation
NETL
NSTL
万方数据
