Experimental study on MnO2 promoting start-up of ANAMMOX process
In order to investigate the promotional effect of MnO2 addition on start-up effect and denitrification performance of ANAMMOX process,by changing hydraulic load and adopting step-by-step domestication means to add MnO2(0-50 mg/L)to the reactor,the tolerance of anaerobic ammonia oxidation sludge to MnO2 was gradually improved and the ANAMMOX reactor was successfully started-up for 90 d of operation.The removal rates of NH4+-N and NO2-N were stabilized at 78%and 98%respectively,the NLR was enhanced to 0.855 kg/(m3·d),the NRR was stabilized at about 0.718 kg/(m3·d),and the mass ratios of NO2--N to NH4+-N and NO3--N to NH4+-N were stabilized at 1.32 and 0.20 respectively,and the maximum value of SAA was 269.86 mg[N]/(g[VSS]·d),and the microbial community structure analysis using high-throughput sequencing technology showed that the genus AnAOB accounted for 8.92%,and became the dominant genus.Compared to the traditional ANAMMOX process,the addition of MnO2 can promote the enrichment of AnAOB and strengthen the activity of AnAOB,which can provide a reference for the wide application of ANAMMOX process in practice.
MnO2ANAMMOXbiological nitrogen removalUASB reactormicrobial community structureAnAOB activity