A rapid start-up strategy based on aeration and dissolved oxygen(DO)combined control was proposed to solve the start-up difficulties and instability of partial nitrification-anammox(PN-Anammox)cou-pling process in practical application.Under the condition of maintaining a low DO environment(＜0.2 mg/L),the oxygen supply of the reaction system was precisely controlled by gradually adjusting the aeration rate(100～175 L/h)to ensure that nitrite nitrogen is provided for the anaerobic ammonia oxidation(Anammox)process,so as to achieve efficient coupling of PN and Anammox.The results showed that when the total nitrogen load of in-fluent water was 533.3 mg/(L·d),the mass concentration of DO was 0.04-0.15 mg/L,and the aeration rate was 175 L/h,the PN-Anammox coupling process showed the best nitrogen removal performance,and the total nitro-gen removal rate reached 77.6％,the removal speed was 423.5 mg/(L·d).In low DO environment,the in situ activities of AOB and AnAOB were significantly enhanced with the increase of aeration rate.After the process was successfully started,the AnAOB activity only reached 51.3％(929.3 mg/(L·d))of the maximum potential,which showed that the system had strong nitrogen removal potential and load shock resistance.In addition,the content of Heme C and the activity of hydrazine dehydrogenase(HDH)were 1.3 and 4.6 times of that of floc sludge,which were 1.21 mmol/g and 0.09 pmol/(g·min)respectively.The addition of carrier is conducive to the growth and enrichment of AnAOB,thereby was beneficial to the stable operation of the reactor.Through the joint control strategy,the PN-anammox coupling process was successfully started in sequence batch reactor(SBR)in only 39 days,and showed excellent nitrogen removal effect in the treatment of high ammonia nitrogen wastewater.
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