Objective To investigate related characteristics of doxorubicin-loaded nanoparticles thermosensitive gel(DOX-NPs-Gel)composite system for hepatic arterial chemoembolization(TACE).Methods Poly-lactic-co-glycolic acid(PLGA)as the carrier,doxorubicin-loaded nanoparticles(DOX-NPs)were prepared by double emulsion.The appearance,particle size,PDI and Zeta potential were investigated;DOX-NPs lyophilized powder was obtained,which was dispersed in chitosan(CS)/β-glycerophosphate(β-GP)thermosensitive gel(Gel)for DOX-NPs-Gel.Its gelling time and gelling temperature of Gel,DOX-Gel,Blank-NPs-Gel and DOX-NPs-Gel were examined by bottle-tumbling assay un the temperature of 37 ℃;microstructure was observed by scanning electron microscope;embolism function of Blank-NPs-Gel was investigated by Rabbit Ear Central Artery Embolism Experiment;subcutaneous injection of Blank-NPs-Gel was administered for mice death by neck dislocation at varied time points,then Blank-NPs-Gel was stripped for measurement and quality in order to evaluate its absorption features.Dynamic membrane dialysis was adopted to examine in vitro release of DOX,DOX-NPs,DOX-Gel;non-membrane dissolution assay was adopted to examine invitro release of DOX-NPs-Gel.Results The prepared DOX-NPs were morphologically regular,with a particle size of(186.68±5.99)nm,PDI of(0.17±0.01),Zeta potential of(-23.33±1.54)mV,encapsulation rate and drug load of(77.10±4.46)％and(2.64±0.19)％.The gelling time of DOX-NPs-Gel at 37 ℃ was(165±15)s;gelling temperature was(34.67±0.47)℃.After lyophilization,its microstructure is neat and tightly porous.The composite system could be successfully injected through a microcatheter,which could successfully embolize the rabbit ear central artery and could be slowly absorbed subcutaneously.In vitro release results showed that the release rates of DOX and DOX-NPs in DOX-NPs-Gel at 7th days were(6.15±0.19)％and(47.25±5.11)％.Conclusion The DOX-NPs-Gel prepared in this study can be rapidly gelling,embolizing blood vessels and be absorbed subcutaneously at body temperature.It can release DOX-NPs slowly.
维普
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