Objective To analyze the effect of dioscin on the proliferation and apoptosis of hepatocarcinoma cell line Bel-7402 and explore its mode of action.Methods Bel-7402 cells were divided into blank group,low-,medium-,and high-dose dioscin groups(given 1,2,8 μmol/L dioscin,respectively)and dioscin+inhibitor group(given 8 µmol/L dioscin+10 µmol/L LY294002(phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)signaling pathway inhibitor)).Tetramethylazolamide(MTT)colorimetry assay was used to detect cell viability at 12,24,36,48,and 72 hours after treatment,and cellular apoptosis was examined at 24 hours using flow cytometry.Western blot was used to detect the expressions of p-PI3K and p-AKT at 24 hours after treatment.Results When compared with blank group,cell viability and expressions of p-PI3K and p-AKT were significantly decreased,while the apoptosis rates were significantly increased in low-,medium-,and high-dose dioscin groups(P＜0.05).Pairwise comparison among each dose group showed statistically significant differences in the levels of the above indicators(P＜0.05).When compared with the high-dose dioscin group,cell viability and the expressions of p-PI3K and p-AKT in dioscin+inhibitor group were significantly decreased,while the apoptosis rate was significantly increased(P＜0.05).Conclusion Dioscin might inhibit proliferation and induce apoptosis of Bel-7402 cells by inhibiting the PI3K/AKT pathway.
维普
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