Objective To investigate the colonization of intestinal flora in normal full-termneonates within 3 months after birth.Methods Clinical data of 20 normal full-term neonates and their pregnant mothers,and fecal specimens were collected from the 1st day(C0),3rd day(C1),1st week(C2),1st month(C3),and 3rd month(C4)after birth.Illumina NovaSeq PE250 was used to sequencing and bioinformatic analysis of 16S ribosomal RNA(16S rRNA)V3+V4 region.Results The number of amplicon sequence variants(ASV)and Shannon index of intestinal flora in normal term neonates were the highest at C0.The postnatal intestinal flora of normal full-term neonates within 3 months after birth was dominated by Proteobacteria,Firmicutes,Actinobacteriota,and Bacteroidota.The relative abundance of Proteobacteria changed from high to low and then gradually increased with age,whereas Firmicutes were the opposite.Within 3 months after birth,the dominant bacteria in normal term neonates were Ralstonia,Streptococcus and Bifidobacterium at the genus level.With the increase of age,the relative abundance of Bifidobacterium increased gradually,reaching 44.18％at C4,and Bifidobacterium was the dominant bacterium.Conclusion The changes take place in diversity,composition and relative abundance of intestinal flora of normal full-term neonates within 3 months after birth.
维普
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